Corilagin对HUVEC增殖及细胞周期的影响

Effects of Corilagin on Proliferation and Cell Cycle of Human Vascular Endothelial Cells

目的研究corilagin对氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)损伤人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVEC)增殖及细胞周期的影响,探索corilagin抗动脉粥样硬化(atherosclerosis,AS)的机理.方法采用ox-LDL复制HUVEC的损伤模型,MTT法观察Corilagin对ox-LDL损伤HUVEC的保护作用.流式细胞术方法分析Corilagin对ox-LDL损伤HUVEC细胞周期的影响.结果与模型对照组比较,Corilagin(6.25μmol/L^50μmol/L),作用12 h、24 h、48 h对HUVEC有保护作用(P<0.05).与模型组比较,corilagin组S期细胞比例增加,G1、G2的变异系数减小,sub-G1减小,其各期细胞分布图趋于正常组的分布比例.结论 Corilagin呈浓度依赖性明显抑制ox-LDL对HUVEC的损伤,其机制可能是通过改善内皮细胞周期的G2/M期阻滞而发挥作用.

Objective To investigate the effects of corilagin on the proliferation and cell cycle of human vascular endothelial cells （HUVEC） stimulated by oxidized low density lipoprotein （ox-LDL）,respectively.Methods The model of injured-HUVEC was duplicated by ox-LDL.The effects of corilagin on ox-LDL-stimulated HUVECS were observed by MTT assay.Flow cytometry （FCM） was used to measure the effects of corilagin on the cell cycle.Results Compared with the model group,the proliferation of HUVECs was significantly increased after treated with corilagin at 6.252 μmol/L-50 μmol/L,incubated respectively for 12 h,24 h and 48 h （P＜0.05）.FCM assay result showed the S-phase cell count of ox-LDL-induced-HUVEC group was reducted significantly,and DNA contant of G2-Phase was increased intensively; S-phase cell count of corilagin incubated HUVEC-injured group increased gradually,meanwhile,each phase of the cell ration had scarcely difference with time changing.Conclusion Corilagin may significantly increase the cell viability of HUVECs,and can protect ox-LDL induced-HUVEC from injury by reieving the G2/M phase arrest of cells.