β-烯醇化酶基因多态性与COPD骨骼肌萎缩的相关性

The Correlation between SNPs in Beta Enolase Gene and Skeletal Muscle Atrophy in patients with COPD

目的探讨β-烯醇化酶基因的2个突变位点:rs121918403(G/A)、rs121918404(G/A)的基因突变率与COPD骨骼肌萎缩的关系.方法采用多聚酶链反应(PCR)及DNA单向直接测序的方法,对昆明地区120例COPD患者和60例年龄匹配正常对照者的2个位点:rs121918403(G/A)、rs121918404(G/A)以及其附近位点进行分析.结果在COPD患者和健康对照组中,rs121918403(G/A)、rs121918404(G/A)2个位点均未发生突变.结论β-烯醇化酶的rs121918403(G/A)、rs121918404(G/A)2个突变位点的基因多态性与昆明地区COPD患者骨骼肌萎缩无相关性.

Objective To investigate the relationship bewteen beta enolase gene two point rs121918403 （G/A）,rs121918404 （G/A） polymorphism with skeletal muscle Atrophy in the patient with chronic obstructive pulmonary disease （COPD） Method Beta enolase genes were assayed by polymerase chain and one-way directed sequencing methods between 120 COPD patients and 60 age-matched controls in Kunming.Result There was no mutation in rs121918403 （G/A） and rs121918404 （G/A） of beta enolase gene in COPD patients and healthy subjects.Conclusion SNPs of two mutational sites of beta enolase are not correlated to skeletal muscle atrophy in COPD patients in Kunming.