GST-π在喉癌及淋巴结微转移中的表达及其临床意义

Expression of GST-π and Significance in Laryngeal Carcinoma and Lymph Node Micrometastasis

目的探讨GST-π基因在喉癌组织中的表达,探讨其相关的临床意义.方法收集昆明医科大学第一附属医院2011年1月至2014年7月手术切除的喉癌标本40例及颈淋巴结组织40枚,另取喉癌手术中的癌旁正常组织40例作为正常对照组.实时定量反转录聚合酶链反应(real-time quantitative reverse transcriptional polymerase chain reaction,real-time)(RT-PCR)技术在喉癌及淋巴结微转移中的检测方法,寻找具有特异性和敏感性的肿瘤标记物.进行HE染色及RT-PCR检测GST-π的表达.结果 GST-π在喉癌组织中的表达高于对照组(P<0.05),喉癌术中进行颈清扫的总的淋巴结组织的表达高于对照组(P<0.05),HE染色阳性的颈淋巴结组织与对照组的表达均有差异(P<0.05).HE染色阴性的颈淋巴结组织中GST-π的表达量与对照组无差异(P>0.05).结论应用real-time(RT-PCR)技术检测喉鳞癌患者颈部淋巴结标本中的GST-π标记物可能是提高检测喉鳞癌和颈淋巴结隐匿性微转移敏感性和特异性的可行方法.

Objective To investigate the real-time quantitative reverse transcriptase polymerase chain reaction （RT-PCR） technique in detecting laryngeal carcinoma and lymph node micrometastasis,find out highly specific and sensitive tumor marker,test GST-π gene expression in laryngeal carcinoma and explore the related clinical significance.Methods We collected 40 cases of resected specimens of laryngeal carcinoma and 40 cases of cervical lymph nodes,and 40 cases of normal tissues as a control group.HE staining and RT-PCR were used for detection of GST-π expression.Results The expression levels of GST-π were higher in laryngeal carcinoma than the control group,in lymph node tissues were higher than control group.The expression in HE staining-positive cervical lymph node was different to control group.The expression GST-π in HE staining-negative cervical lymph node tissue had no difference with the control group.Concltusions GST-π may be associated with the development of laryngeal carcinoma,may become one of the indicators of detection of micrometastases in cervical lymph nodes.Application of real-time （RT-PCR） for detection of GST-π marker in laryngeal squamous cell carcinoma and neck lymph node specimens may improve the sensitivity and specificity in detection of laryngeal squamous cell carcinoma micrometastasis.