解脲支原体、沙眼衣原体和巨细胞病毒感染与输卵管妊娠的相关性研究

Correlation between the Tubal Pregnancy and Ureaplasma Urealyticum, Chlamydia Trachomatis and Humancytomegalovirus Infection

目的探讨解脲支原体(UU)、沙眼衣原体(CT)及人巨细胞病毒(HCMV)感染情况与输卵管妊娠(TP)的关系.方法应用PCR技术检测62例TP患者(病例组)和48例非TP患者(对照组)的宫颈分泌物和输卵管中的CT、UU、HCMV的DNA.结果 (1)病例组中输卵管标本的CT、UU、HCMV单项检出率、CT+UU及UU+HCMV合并感染率均高于对照组,两者比较有显著性差异(P<0.05);(2)病例组中宫颈分泌物标本的CT、UU、HCMV单项检出率、CT+UU及CT+UU+HCMV合并感染率均高于对照组,二者比较有显著性差异(P<0.05);(3)两次以上TP患者输卵管标本及宫颈分泌物标本的CT、UU、HCMV单项检出率高于仅有一次TP患者,二者比较有显著性差异(P<0.05).结论 (1)TP与CT、UU及HCMV感染有一定关系,可作为TP发生的可能性的检测指标;(2)两次以上TP患者的CT、UU、HCMV检出率明显增高,TP患者在排除其他因素的同时应考虑病原体感染的可能,积极治疗及预防感染,以免再次TP的发生;(3)聚合酶链反应(PCR)具有简单、快捷、准确性高的特点,可以作为临床早期病原学诊断依据.

Objective To explore the relationship between tubal pregnancy and ureaplasma urealyticum （UU）,chlamydozoa trachomatis （CT） and Human Cytomegalovirus （HCMV） infection.Methods 62 patients with tubal pregnancy （study group） and 48 patients with non-tubal pregnancy （control group） were recruited in this study.Cervical secretion and fallopian tube samples were collected to detect CT-DNA,UU-DNA and HCMV-DNA using Polymerase chain reaction （PCR） Results （1） In the tubal pregnancy group,the single positive rates of CT-DNA,UU-DNA,HCMV-DNA and the joint positive rates of CT＋UU-DNA,UU＋HCMV-DNA in the fallopian tube samples were significantly higher than those in the control group （P＜0.05）.2.In the tubal pregnancy group,the single positive rates of CT-DNA,UU-DNA,HCMV-DNA and the joint positive rates of CT＋UU-DNA,CT＋UU＋HCMV-DNA in the cervical secretion samples were significantly higher than those in the control group （P＜0.05）.3.In the patients who had more than twice tubal pregnancy,the single positive rates of CT-DNA、UU-DNA、HCMV-DNA in the fallopian tube samples and cervical secretion samples were significantly higher than those in the patients who had only once tubal pregnancy （P＜0.05）.Conclusion Tubal pregnancy is related to the infection of CT,UU and HCMV,which may be used as a predictive marker of tubal pregnancy.PCR is simple,rapid and accurate in detection of CT,UU and HCMV infection.