富氢水抗1型糖尿病小鼠肾脏氧化应激的作用

Antioxidation of hydrogen-rich water on renal injury induced by type 1 dibetes in mice

目的:研究富氢水对糖尿病小鼠肾脏的保护作用,并探讨其可能的作用机制。方法:60只健康ICR雄性小鼠,随机分成空白对照组(A组)、糖尿病组(B组)和糖尿病富氢水干预组(C组)。链脲佐菌素(STZ)150 mg/kg腹腔注射诱发糖尿病,造模成功后,C组每日腹腔注射富氢水5 m L/kg,B组腹腔注射等量0.9%氯化钠溶液,连续8周。尾静脉取血测定小鼠血糖,HE染色观察肾脏组织学变化,免疫组织化学染色观察NADPH氧化酶4(Nox4)和血红素加氧酶-1(HO-1)蛋白的表达。结果:C组Nox4表达水平明显低于B组,而HO-1表达明显高于B组;B组与C组的空腹血糖均明显高于A组(P<0.01),B组与C组之间差异无统计学意义(P>0.05);3组肾脏组织病理HE染色未显示明显病变。结论:富氢水可减轻糖尿病小鼠肾脏组织的氧化应激水平。

Objective： To study the protective effect of hydrogen on renal injury induced by type 1 dibetes in mice and explore the possible mechanism.Methods： Sixty healthy male ICR mice were randomly divided into control group （A）, diabetic group （B） and diabetes hydrogen water intervention group （C）. Diabetes were induced by intraperitoneal injection of streptozotocin 150 mg/kg. Group C were injected saturated hydrogen-rich water 5 mL/kg and group B were injected with equal normal saline every day for 8 weeks in mice models of type 1 dibetes. The blood was collected from the tail vein and blood glucose was measured. Renal injury were identiifed by hematoxylin and eosin （HE） staining. Expression of Nox4 and HO-1 were identiifed by immuno-histochemical staining.Results： Expression of Nox4 in Group C was signiifcantly lower than that in group B, expression of HO-1 in Group C was signiifcantly higher than that in group B. Blood glucose in Group B and C were higher than that in Group A, but there was no difference between the two groups. Kidney HE staining did not show signiifcant lesions.Conclusion： Hydrogen-rich water can reduce oxidative stress in renal tissue of dia-betic mice.