摘要
目的联合检测p27、p53、p21、环氧化酶-2(cyclooxygenase-2,COX-2)、表皮生长因子受体(epithelial growth factor receptor,EGFR)在喉鳞状细胞癌(简称喉鳞癌)的表达,为喉癌的诊断及治疗提供分子生物学依据。方法 96例喉鳞癌石蜡切片,应用免疫组织化学方法检测p27、p53、p21、COX-2及EGFR在喉鳞癌组织的表达水平。结果 p27、p53、p21、COX-2及EGFR在原发性喉鳞癌组织中的阳性表达率分别为67.71%(65/96)、70.83%(68/96)、88.54%(85/96)、75.00%(72/96)及96.88%(93/96)。p27的表达与肿瘤病理分化及淋巴结转移有关,中-低分化组与高分化组、淋巴转移组与未转移组间表达有统计学差异。COX-2的表达与患者的年龄组表达有关,年龄组间表达有统计学差异。结论 p27表达水平在喉癌的发生发展中起重要作用。
OBJECTIVE To study the expression and their clinical significance of p27, p53, p21,cyclooxygenase-2（COX-2） and epithelial growth factor receptor（EGFR） in laryngeal squamous cell carcinoma. METHODS Expression of p27, p53, p21, COX-2 and EGFR were evaluated in specimens of 96 primary laryngeal squamous cell carcinoma patients by immunohistochemistry. RESULTS The positive expression rates of p27, p53, p21, COX-2 and EGFR in laryngeal squamous cell carcinoma were 67.71%（65/96）, 70.83%（68/96）, 88.54%（85/96）, 75.00%（72/96） and 96.88%（93/96） respectively. There were significant positive correlation between p27 with pathological differentiation and negative correlation with lymph node metastasis. There was significant difference in the expression of p27 among different differentiation groups and between the lymph node metastasis group and no lymph node metastasis group. There was significant difference in the expression of COX-2 among different age groups. CONCLUSION p27 may serve as a vital indicator for the treatment or prognosis of primary laryngeal squamous cell carcinoma.
出处
《中国耳鼻咽喉头颈外科》
CSCD
2014年第7期353-356,共4页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
广东省自然科技基金资助项目(S2013010014632)
