基于HepG2、SMMC-7721肝癌细胞生长抑制的鸡血藤总黄酮纯化前后药效对比研究

Comparative Efficacy before and after Purification of Total Flavonoids in Millettla Based on the Growth Inhibition of HepG2 and SMMC-7721 Hepatic Cell

目的:探讨鸡血藤总黄酮纯化前后对HepG2和SMMC-7721肝癌细胞药效对比的影响;明确鸡血藤抗肝癌作用的物质基础。方法:以肝癌细胞抑制率为评价指标,将纯化前后不同剂量的鸡血藤总黄酮分别作用于HepG2和SMMC-7721两株肝癌细胞24h、36h、48h后,采用MTT法检测其对两株肝癌细胞抑制情况。结果:鸡血藤纯化前后的总黄酮均能够显著抑制肝癌HepG2和SMMC-7721肝癌细胞的增殖。相同给药时间内,纯化后的鸡血藤总黄酮对肝癌细胞的抑制率均大于未纯化的鸡血藤黄酮抑制率。此外,无论纯化前与后,其抗肝癌作用随着时间和剂量的增加而增强,呈现显著的时-效、量-效关系(P<0.05)。结论:通过体外药效学比较,显示鸡血藤总黄酮纯化后药效优于纯化前药效,表明鸡血藤总黄酮是鸡血藤抗肝癌的主要物质基础,为鸡血藤在治疗肝癌方面的发展及临床合理利用奠定基础。

Objective： Discussion the pharmacodynamic efficacy before and after purification of total flavonoids in Millettla based on the growth inhibition of HepG2 and SMMC-7721 in liver tumors; Be clear the anti-hepatoma effect of Millettla basis material. Methods ： s： Evaluation by Hepatoma cells＇ Inhibition rate, The total different doses of flavonoids in Millettla before and after purified was given to the two strains of HepG2 and SMMC-7721 hepatoma cells 24h.36h.48h. Detect inhibition of two strains of hepatoma cells by MTT assay. Results：Total flavonoids in Millettla before and after purified were able to inhibit HepG2 and SMMC-7721 cell proliferate significantly. At the same time within the administration, Millettla after purification of total flavonoids inhibit cancer rates greater than unpurified Millettla flavonoids inhibition. In addition,Whether before or after purification, Its anti-liver cancer effects enhanced with the increasing doses and time, Showed a significant time-effect and dose-effect relationship（P〈0.05）. Conclusion：By the comparison in vitro pharmacodynamic,After purification of total flavonoids Millettla display superior efficacy before purification efficacy,Show Millettla total flavonoids are the main material basis Millettla anti-liver cancer, Lay the foundation for Millettla development in the treatment of liver cancer and the clinical aspects of rational use.