推拿手法对家兔失神经支配后肌球蛋白重链mRNA表达的影响

Changes of Myosin Heavy Chain mRNA in Muscle Atrophy after Tuina Therapy in Rabbits

目的:探讨推拿手法延缓周围神经损伤后失神经肌萎缩的作用效应和途径。方法:将失神经支配的家兔90只随机分为手法治疗组42只、模型对照组42只和正常组6只,手法治疗组给予按揉法治疗,模型对照组与正常组不做处理。分别在造模后第1、2、3周和第1、2、4、6个月逐月各取1小组6只家兔检测肌球蛋白重链mRNA表达。结果:手法治疗组与模型对照组比较,肌球蛋白重链MHC-Ⅱ亚型在1周、2周时明显升高,MHC-Ⅰ亚型在3周、1月、2月时明显升高,统计学上有显著性差异(P<0.05)。结论:推拿手法促进肌肉结构改建能力,促进骨骼肌再生修复。

Objective： To observe the changes of myosin heavy chain mRNA in muscle atrophy after Tuina therapy.Method：90 male rabbits with denervated skeletal muscle were randomly divided into the normal group,control group andtreatment group. The changes of myosin heavy chain mRNA were detected 1,2 and 3 weeks after modeling and 1,2,4 and 6 months by selecting 6 rats in each group. Result ： MHC -Ⅱ between treatment group and control group had statisticaldifferences （ P 〈 0.05） at 1 and 2 weeks and MHC - I between treatment group and control group had statistical differ-ences （ P 〈 0.05 ） at 3 weeks, 1 months and 2 months. Conclusion ： Tuina therapy can promote reconstructing of muscleand delay muscle atrophy of denervated skeletal muscle.