摘要
目的:探讨痔愈喷剂对大鼠术后创面愈合的作用机制。方法:30只SD大鼠随机分为痔愈喷剂组、空白对照组、湿润烧伤膏组。按照制备大鼠皮肤创伤模型,分别于造模后第7、14、21天,取创伤周围少量皮肤组织。采用免疫组织化学技术观察创面肉芽组织中TGF-β1表达的变化。结果:造模术后第7、14、21天痔愈喷剂组TGF-β1含量明显高于空白对照组(P<0.05),与湿润烧伤膏组相比无明显差异。结论:痔愈喷剂可增加术后创面TGF-β1表达量,促进创面愈合的作用。
Objective：To investigate the mechanism of hemorrhoid healing sprays on postoperative wound healing inrats. Methods ：30 SD rats were randomly divided into hemorrhoid healing sprays group, blank control group and MEBO group. Full - thickness skin defects with rat back trauma were made. And then a small amount of skin tissue around the wound was taken out in 7 days,14 days and 21 days. In the model after 7 days,14 days and 21 days,we observed each wound healing by using image analysis software to measure each wound area and immunohistochemical method to detect TGF-β1 content in granulation tissue. Results： After surgery,7 days 14 days and 21 days hemorrhoid healing spray group＇ s TGF - β1 was significantly higher than that of the control group （ P 〈 0.05 ）. There was no significant difference compared with MEBO group. Conclusion：Hemorrhoid healing sprays can increase the expression of TGF - β1 ＂after surgery and promote wound healing.
出处
《中华中医药学刊》
CAS
北大核心
2015年第1期165-167,I0017,I0018,共5页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药优秀青年人才基金计划项目(2012ZQ019)
温州市科技局项目(Y20140009)
