猫爪草总皂苷对人非小细胞肺癌A549细胞裸鼠移植瘤生长及EGFR、MMP-9表达的影响

Effect of Radix Ranunculus Ternati Saponins on Growth of Human Non-Small Cell Lung Cancer of Cell A549 in Nude Mice and EGFR and MMP-9 Expression

目的:观察猫爪草总皂苷对人非小细胞肺癌A549细胞裸鼠移植瘤的抑制作用,及对表皮生长因子受体(EGFR)、基质金属蛋白酶-9(MMP-9)表达的影响。方法:建立A549裸鼠移植瘤模型,待肿瘤长到100mm3以上,开始分组、称重、给药,分别设猫爪草总皂苷高剂量组(1.73 g/kg)、中剂量组(0.86 g/kg)、低剂量组(0.43 g/kg),同时设环磷酰胺(CTX)阳性对照组(30 mg/kg×3)及水阴性对照组(30 m L/kg),每隔3 d测量肿瘤体积,绘制肿瘤生长曲线图;18 d后脱颈处死裸鼠,称瘤重,计算抑瘤率,病理常规HE染色,免疫组化法检测EGFR、MMP-9在瘤组织中的表达。结果:各治疗组移植瘤生长较水对照组缓慢,猫爪草总皂苷高剂量组瘤重与水对照组比较差异显著(P<0.05),不同剂量猫爪草总皂苷的抑瘤率为13.8%-32.6%。猫爪草总皂苷高剂量组14 d和18 d的肿瘤生长体积、中剂量组18 d的肿瘤生长体积与水对照组相比差异显著(P<0.05)。不同剂量猫爪草总皂苷组的EGFR、MMP-9的阳性表达率与水对照组相比有所降低,且呈剂量依赖性,其中高剂量组的EGFR阳性表达率与水对照组相比差异显著(P<0.05)。结论:猫爪草总皂苷能较好地抑制A549裸鼠移植瘤生长,并下调瘤组织EGFR、MMP-9的表达。

Objective ： To observe the effect of Radix Ranuneulus Ternati Sapouins （RRTS） on the growth of non -small cell lung cancer （NSCLC） of cell A549 in nude mice and EGFR and MMP - 9 expressions. Methods ： A model oftransplanted tumors bearing A549 cells was established in nude mice. After the tumors grew to more than 100 mm3 ,micewere divided into five groups：high （1.73 g/kg） ,middle （0.86 g/kg） and low （0.43 g/kg） doses of RRTS,CTX group（ 30 mg/kg x 3 ） and control group. Tumor volume was measured every three days and tumor growth curves were marked.Mice were sacrificed by neck luxation after 18 days. According to the final tumor weight, the inhibition rate was calculat-ed. Pathological changes were observed by HE staining. The expressions of EGFR and MMP - 9 were inspected by immu-nohistochemical method. Results ： The growth of transplanted tumors in treatment groups was significantly slowed than thatof control group. The tumor weight of high close of RRTS group was smaller than that of control group（ P 〈 0.05 ）. The in-hibition rate in three dose groups was between 13.8% and 32.6%. The tumor volume of 14 d and 18 d of high dosegroup and 18d of middle dose group were lowered than that of control group（ P 〈 0.05 ）. Conclusion ： RRTS can inhibit thegrowth of human lung adenoearcinoma cell A549 in nude mice and lower expressions of EGFR and MMP - 9.