COX-2、TTF-1及p63蛋白在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of COX- 2,TTF- 1 and p63 in non- small cell lung cancer

目的:研究环氧化酶-2(COX-2)、甲状腺转录因子-1(TTF-1)及p63蛋白在非小细胞肺癌(NSCLC)中的表达情况,并探讨其与NSCLC病理类型、临床TNM分期及淋巴结转移之间的关系。方法:采用免疫组化法检测156例NSCLC患者术后病理组织中COX-2、TTF-1、p63蛋白的表达水平。结果:COX-2、TTF-1及p63蛋白在NSCLC组织中的阳性表达率分别为94.5%(137/145)、61.0%(72/118)、53.5%(54/101)。在肺腺癌和鳞癌中,COX-2阳性表达率无显著性差异(P>0.05),而TTF-1和p63蛋白阳性表达率具有显著统计学意义(P<0.001)。COX-2和p63蛋白阳性表达率与NSCLC患者TNM临床分期、淋巴结转移均无统计学差异(P>0.05)。在Ⅲ-Ⅳ期或有淋巴结转移的NSCLC病人中,TTF-1阳性表达率显著高于Ⅰ-Ⅱ期或无淋巴结转移的病人(P<0.01)。结论:在NSCLC病人中,TTF-1和p63蛋白的表达可能分别是腺癌和鳞癌的有效生物标志物,TTF-1的阳性表达可能与肺癌的浸润、侵袭和转移相关。

Objective：To study the expressions of cyclooxygenase-2（ COX-2 ）,thyroid transcription factor-1 （TTF-1）and p63 protein in non-small cell lung cancer（NSCLC）as well as the relationship between their expres-sions and pathological type,clinical TNM stage and lymph node metastasis. Methods：The expression level of COX-2,TTF-1 and p63 protein were detected by immunohistochemistry in postoperative pathological tissues of 156 NSCLC patients. Results：In NSCLC patients tissues,the positive expression rates of COX-2,TTF-1 and p63 protein were 94. 5%（137/145）,61. 0%（72/118）,53. 5%（54/101）,respectively. COX-2 expression was no significant differ-ence between adenocarcinoma and squamous carcinoma（p〉0. 05）,while the positive expression rates of TTF-1 and p63 protein were different（p〈0. 001）. The expression rates of COX-2 and p63 protein were not associated with NSCLC TNM clinical stage and lymph node metastasis（p〉0. 05）. In NSCLC patients with stage Ⅲ-Ⅳ or lymphatic metastasis,the positive expression rate of TTF-1 was significantly higher than that in stage Ⅰ -Ⅱ or without lymphatic metastasis patients（p〈0. 01）. Conclusion：The expression of TTF-1 and p63 protein might be effective biomarkers of adenocarcinoma and squamous carcinoma in NSCLC patients. The positive expression of TTF-1 might be related to infiltration,invasion and metastasis of lung cancer.