樱桃谷种鸭鸭1型甲肝病毒的分离鉴定及其VP1基因的序列分析

Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck

从某樱桃谷种鸭场病死樱桃谷种鸭的肝脏中分离到1株病毒(命名为HeN1403),该病毒能在79h内致死10日龄番鸭胚,对8日龄雏番鸭的致死率为33.3%。应用鸭1型甲肝病毒特异性引物对HeN1403分离株进行RT-PCR检测,可扩增到约200bp的目的条带,并对分离株的VP1基因进行扩增,得到714bp的基因片段。通过软件对分离株VP1基因序列与GenBank上发表的12株不同基因型鸭1型甲肝病毒的VP1基因核苷酸序列的同源性进行比对和遗传进化分析,确定该分离株为鸭1型甲肝病毒。

A virus strain,named HeN1403,was isolated from the liver of dead Cherry Valley Breeding ducks .The virus killed 10 days Muscovy duck embryo within 79 hours and showed 33.3% of mortality rate to eight-day-old Muscovy ducklings.HeN1403 was characterized by specific RT-PCR primers which target to duck hepatitis virus A type 1,and it turned out to be a 200 bp fragment.A 714-bp fragment gene was obtained by using a set of primers aimed at VP1 ORF gene.Genetic analysis showed that the isolated virus was one of pancreatitis-type duck hepatitis virus A type 1.