植物激素对果蔗组培苗分蘖的影响

The Effect of Plant Hormones on Tillering of Chewing Cane Somaclone

以未组织培养的果蔗种苗为对照,对组培苗后代4种内源激素进行测定分析,以及通过外源激素处理研究果蔗分蘖机理和调控技术。结果显示:组培苗后代中IAA和CTK含量比对照高,它们可能在分蘖过程中起作用,ABA和GA3含量与对照无明显差异;外源激素处理显示,6-BA处理后可以显著提高果蔗的分蘖率,而NAA对抑制果蔗分蘖有一定的效果。综合内源激素测定和外源激素喷施的结果,推测影响果蔗组培苗分蘖的因素可能是组培过程中残留的6-BA在起作用。

With no tissue culture chewing cane seedling was used as control,4 kinds of endogenous hormones of the somaclone offspring were tested,and the mechanism of tillering of chewing cane and control technology was studied by exogenous hormone treatment.Results showed that IAA and CTK content of somaclone offspring were higher than control,which suggested that they might play a role in the process of tillering,but ABA and GA3 content was not significantly different from control.The result of exogenous hormone treatment showed that 6-BA treatment could significantly improve tillering rate of chewing cane,and NAA had the certain effect on the inhibition of chewing cane tillering.Comprehensive the result above,we speculated that the effect factors of chewing cane somaclone tillering might be the 6-BA which was remained in the process of tissue culture.