栀子叶绿体DNA条形码的研究

Identification of Gardenia Based on cpDNA Barcoding

目的评价3个叶绿体DNA(cpDNA)条形码候选序列对茜草科植物栀子属的鉴别能力,探索栀子属植物鉴定的新方法。方法使用matK、rbcL和psbA候选序列的通用引物对栀子属植物cpDNA进行PCR扩增和测序,比较各序列的扩增成功率、长度、种内和种间变异、barcoding gap,并采用BLAST和DNA MAN进行分析评价。结果 matK、rbcL、psbA序列对栀子属3个物种、5个样本的扩增成功率均为100%,其中通用引物matK扩增的序列种内种间变异差异、barcoding gap较psbA、rbcL序列具有更明显的优势,其鉴定成功率相对较高。结论 matK是适合栀子属植物鉴别的较好cpDNA条形码。

Objective To test and eva1uate the abi1ity of three potential chloroplast DNA （cpDNA）barcoding sequences;To find new methods to identify the species of gardenia. Methods ThreecpDNA sequences were amplified and sequenced by universal primers of matK, rbcL and psbA. Bycomparing PCR amplification efficiency, length, intra- and inter-specific divergence, and barcodinggap, BLAST and DNA MAN were used to evaluate these loci. Results The amplification efficiency of 5samples from 3 gardenia species was 100%. Analysis of the intra- and inter-specific divergence ofmatK among the sequences showed that barcoding gap was superior to psbA and rbcL, with higheridentification efficiency. Conclusion Gardenia jasminoides Ellis can be better identified by matKsequence.