肝硬化及肝细胞癌组织中阻抑蛋白表达的比较

Compare of Expression of PHB mRNA in tissues of hepatocellular carcinoma and cirrhotic liver

目的了解阻抑蛋白 （prohibitin, PHB）mRNA 在肝癌组织、肝硬化组织及正常对照组织中的表达及关系. 方法： 采用逆转录-聚合酶链反应方法检测PHBmRNA 在45 例肝细胞癌（hepatocellular carcinoma,HCC）患者肝癌组织、癌旁肝硬化组织、非肝癌的肝硬化患者活检组织、肝血管瘤及肝内胆管结石等对照组组织中的表达,用捷达801 分析软件对结果进行相对定量分析.结果： PHB mRNA 表达率在肝癌组织、肝硬化组织对照组分别为70%、84.1%、100%,表达率之间无明显统计学差异（ P〉0.05）;经定量分析,肝癌组织、肝硬化组织和对照组PHB mRNA 表达量分别为0.81±0.57、1.16±0.58、1.97± 1.24; 扩增产物表现出表达量的不同,呈现渐变趋势,HCC 组织、肝硬化组织与对照组之间明显统计学差异（ P 〈0.05）,HCC 组织与LC 组织比较无统计学差异（ P 〉0.05）.结论：在肝硬化、肝癌的发生发展过程中,PHB 基因可能是肝硬化和HCC 潜在诊断标志物和治疗靶点.

AIM To investigate the expression of prohibitin ） mRNA and its relationship in hepatocellular carcinoma, cirrhotic liver and normal liver tissues. METHODS：Detection of the expression of PHB mRNA in 45 specimens of hepatocellular carcinoma, cirrhotic liver, normal liver tissues were done by RT-PCR, and then relative quantificationwas analyzed by a software of Jie-Da 801. RESULTS： The expression rate of PHB mRNA in hepatocellular carcinoma, cirrhotic liver and normal liver tissues were 70%, 84.1% and100% respectively, but there’re no significant differences between them （P 〉 0.05）. The relative Quantification of expression level of PHB mRNA were 0.81±0.57, 1.16±0.58,1.97±1.24 respectively, which was significantly lower in hepatocellular carcinoma and cirrhotic liver tissues than that in normal liver tissues （P〈0.05）. CONCLUSION： PHB genemay be a potential biomarker for diagnosis and target for therapy in the progression of hepatocellular carcinoma and cirrhotic liver.