2型糖尿病患者肠道乳酸菌属和消化链球菌属定量研究及其意义

Significance of the quantitative analysis of Lactobacillus and Peptostreptococcus productus of gut microbiota in patients with type 2 diabetes

目的对2型糖尿病患者与健康人肠道乳酸菌属和消化链球菌属进行定量分析,探讨两组人群肠道菌群的差异。方法提取肠道菌群总DNA,利用菌属特异性引物实时荧光定量PCR扩增,制作菌属标准品和标准曲线,定量分析两种优势菌属在糖尿病患者和健康人群中的含量。结果与健康对照组相比,2型糖尿病组肠道消化链球菌的数量均显著减少,而乳酸菌属的数量两组间无统计学差异。结论荧光定量PCR方法可作为研究肠道菌群的一种准确、敏感的检测方法;糖尿病患者肠道优势菌属发生变化,提示肠道优势菌群可能与糖尿病的发生发展有一定的相关性。

Objective To analyze quantitatively the intestinal Lactobacillus genus and Peptostreptococcus productus of patients with type 2 diabetes and healthy people to compare the differences of intestinal flora between the two groups of people. Methods The total DNA of intestinal microbiota was extracted. The specific primers of bacterial genus were used for real-time fluorescent quantitative PCR amplification. And bacterial standard and standard curve were made. Then we analyzed quantitatively the content of two predominant bacterial genus in patients with type 2 diabetes and healthy people. Results Compared with that in the healthy controls, the number of the intestinal Peptostreptococcus productus in the patients with type 2 diabetes was significantly reduced. However, as for the number of intestinal Lactobacillus genus, no significant differences were found between the two groups. Conclusion Real-time PCR can be taken as an accurate and sensitive testing method to study the intestinal microbiota. The changes of the intestinal predominant bacterial genus in patients with diabetes suggest a certain correlation of the intestinal predominant bacterial genus with the occurrence and development of diabetes.