蝉翼藤中甲基阿魏酸对CCl_4致急性肝损伤小鼠模型的影响

The Influence of Methyl Ferulic Acid of Securidaca inappendiculata on Acute Liver Injury Induced by CCl_4 in Mice

目的:研究蝉翼藤中甲基阿魏酸对急性肝损伤模型小鼠的保护作用。方法:60只KM小鼠随机均分为正常对照(等体积淀粉浆)组、模型(等体积淀粉浆)组、联苯双酯(100 mg/kg)组与甲基阿魏酸高、中、低剂量(200、100、50 mg/kg)组,灌胃给药,每天1次,连续7 d。末次给药1 h后,一次性腹腔注射0.5%CCl4-花生油(10 ml/kg)以复制小鼠急性肝损伤模型。复制模型24 h后,测定小鼠肝脏、脾脏指数,测定小鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性,测定小鼠肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性与丙二醛(MDA)含量,光镜观察小鼠肝脏病理组织学并统计肝组织病变分级。结果:与正常对照组比较,模型组小鼠肝脏、脾脏指数升高;小鼠血清ALT、AST活性增强;小鼠肝组织SOD、GSH-Px活性减弱,MDA含量增加;小鼠肝组织全部出现病变,多集中于Ⅲ级,差异均具有统计学意义(P<0.01)。与模型组比较,甲基阿魏酸高、中、低剂量组小鼠肝脏、脾脏指数降低;小鼠血清ALT、AST活性减弱;小鼠肝组织SOD、GSH-Px活性增强,MDA含量减少;甲基阿魏酸高、中剂量组小鼠肝组织病变程度减轻,多集中于0、Ⅰ、Ⅱ级,差异均具有统计学意义(P<0.01或P<0.05)。结论:甲基阿魏酸对CCl4所致小鼠急性肝损伤有一定的保护作用。

OBJECTIVE： To observe the influence of methyl ferulic acid （MFA） ofSecuridaca inappendiculata on acute hepatic injury in mice induced by CCL. METHODS： 60 mice were randomly divided into 6 groups as normal control group （constant volume of starch paste）, model group （constant volume of starch paste）, bifendate group （100 mg/kg）, MFA high dose group （200 mg/kg）,MFA middle dose group（100 mg/kg）, MFA low dose group（50 mg/kg）. They were given relevant medicines once a day for consecutive 7 days. On the seventh day after administration for 1 hour, except the normal group, the other groups were intraperitoneally injected with 0.5% CCL peanut oil 10 ml/kg. After 24 hours of duplicating model, liver index, spleen index and the serum activities of ALT and AST were determined respectively. SOD and GSH-Px activities and MDA content in liver mitochondria were also determined. Liver tissue sections were examined under light microscope. RESULTS： Compared with normal control group, the liver index and spleen index increased in model group, and the serum levels of ALT and AST increased, the activity of SOD and GSP-Px decreased, the content of MDA increased; all the lesions appeared in liver tissue of mice, and more concentrated in the class llI, there were statistic significant difference （P〈0.01）. Compared with model group, the liver index and spleen index decreased in MFA hig-dose, medium-dose, low-dose groups, and the serum levels of ALT and AST decreased, the activity of SOD and GSP-Px increased, the content of MDA decreased; the degree of liver tissue of mice alleviated in MFA high-dose and medi- um-dose groups, focused more on class 0, ⅠandⅡ . There were statistic significant difference （P〈0.01 or P〈0.05）. CONCLUSIONS ： MFA has protective effect on acute liver injury by CC14 in mice induced.