超声联合携抗ICAM-1微泡的双靶向载体系统增强基因转染受损内皮细胞

Double Targeting Carrier System Ultrasound in Combination with Microbubbles Comprising Anti-ICAM-1 Cation for Enhancement of Gene Transfection in Damaged Endothelial Cells

目的:探讨超声联合携抗细胞间黏附分子(ICAM)-1阳离子微泡的双靶向载体系统介导基质细胞衍生因子(SDF)-1α基因转染受损内皮的效果。方法:实验分组,A组:携SDF-1α基因抗ICAM-1阳离子微泡;B组:携SDF-1α阳离子微泡;C组:空白对照。体外实验:人白细胞介素-1β刺激制备大量表达ICAM-1的血管内皮细胞模型,各组联合超声辐照介导基因转染后测定基因转染率、基因和蛋白表达情况。体内实验:结扎冠状动脉前降支制备兔心肌梗死模型,超声辐照介导基因转染后取心肌组织,Western blot检测各组心肌SDF-1蛋白的表达情况,RT-PCR检测各组SDF-1 mRNA的表达情况。结果:无论是体外实验还是体内实验,A组:携SDF-1α基因抗ICAM-1阳离子微泡组超声辐照后的基因转染率、hSDF-1α基因mRNA表达和hSDF-1α蛋白表达情况均高于其他各组。结论:超声联合携抗ICAM-1微泡的双靶向载体系统能增强SDF-1α基因转染受损内皮细胞。

Objective：To investigate the effect of double targeting carrier system ultrasound in combination with microbubbles comprising anti-ICAM-1cation mediating SDF-1α gene transfection in damaged endothelial cells.Methods：There were three groups,including the microbubbles comprising SDF-1αgene and anti-ICAM-1cation group（group A）,the microbubbles comprising SDF-1αgene group（group B）,and the blank control group（group C）.In vitro,the vascular endothelial cell model with a large number of ICAM-1expression was established by the stimulation of human interleukin-1β;after gene transfection viaultrasonic irradiation mediating,the efficiency of gene transfection was detected by flow cytometry;the SDF-1αmRNA expression was measured by PCR,and the SDF-1αprotein expression was determined by Western Blot.In vivo,therabbit model of myocardial infarction was established by ligation of anterior descending coronary artery;after gene transfection by ultrasonic irradiation mediating,the SDF-1αprotein and mRNA expression in myocardial tissues was detected by Western blot and RT-PCR.Results：After gene transfection viaultrasonic irradiation mediating,the efficiency of gene transfection and the expression of SDF-1αmRNA and protein in group A（microbubbles comprising SDF-1αgene and anti-ICAM-1cation group）were all higher than other groups in vitro and in vivo.Conclusion：Double targeting carrier system ultrasound in combination with microbubbles comprising antiICAM-1cation can enhance SDF-1αgene transfection in damaged endothelial cells.