β神经生长因子对内皮祖细胞一氧化氮合酶的影响

Influence of Beta-Nerve Growth Factor on the Production of eNOS and NO by Endothelial Progenitor Cells

目的:探讨β-神经生长因子(β-NGF)对大鼠内皮祖细胞(EPCs)内皮型一氧化氮合酶(eNOS)及一氧化氮(NO)合成的影响。方法:将β-NGF重组腺病毒(Ad-β-NGF)及其阴性对照感染至大鼠内皮祖细胞,同时设置空白对照,分别于24h、48h及72h进行检测。用qPCR及Western blot分别在mRNA和蛋白水平检测不同组eNOS表达情况,硝酸还原酶法测定培养液中NO的水平。结果:Ad-NGF感染组eNOS表达及NO合成明显高于对照组,差异有统计学意义(P<0.05),并随感染时间的延长而增加。结论:β-NGF能促进内皮祖细胞eNOS表达及NO合成,有利于内皮祖细胞发挥功能。

Objective：To evaluate the effect ofβ-nerve growth factor（NGF）on the production of endothelial nitric oxide synthase（eNOS）and NO by endothelial progenitor cells（EPCs）.Methods：Primary rat EPCs were isolated from rat bone marrow.The purified EPCs were infected with AdEGFP-β-NGF and Ad-EGFP respectively,an addition pseudo-infection was acted as blank control.The effect was observed after infection at 24 h,48hand 72 h,respectively.The level of eNOS was tested by both q-PCR and Western-blot assay.The content of NO was determined by nitrate reductase.Results：The expression of eNOS and the production of NO increased greatly（P〈0.05）compared with control group,which exhibited a time-dependent manner.Conclusion：β-NGF could improve the function of EPCs by promoting the expression of eNOS and NO.