TGR7 mRNA在肺癌石蜡组织中的表达及临床意义

Expression of TGR7 mRNA in Paraffin-embedded Lung Cancer Tissue and Its Clinical Significance

目的:探讨福尔马林固定石蜡包埋的非小细胞肺癌(Non-small-cell lung cancer,NSCLC)中Mas相关的G蛋白偶联受体TGR7的表达及其临床意义。方法:提取125例NSCLC及其对应癌旁肺组织的mRNA,使用实时荧光定量RT-qPCR检测TGR7的表达,分析其与临床各病理参数的关系。结果:TGR7 mRNA在NSCLC组织中的表达(1.068 2±0.609 6)明显高于癌旁肺组织(0.399 4±0.283 8),P<0.001。TGR7 mRNA表达在淋巴结转移组(1.201 0±0.633 4)明显高于非淋巴结转移组(0.904 5±0.540 6),P=0.006,在肿瘤直径≥3cm组(1.193 5±0.589 5)明显高于肿瘤直径<3cm组(0.932 3±0.606 6),P=0.016,在TNM临床Ⅲ、Ⅳ期(1.176 2±0.632 9)明显高于Ⅰ、Ⅱ期(0.926 1±0.551 5),P=0.022。结论:TGR7mRNA的高表达与NSCLC发生与进展密切相关,可望作为NSCLC的促癌基因及成为NSCLC诊断、预后和靶向治疗的指标。

Objective ：To investigate the expression and clinical significance of Mas related G Protein‐Coupled Receptor TGR7 in non‐small cell lung cancer （NSCLC） formalin‐fixed paraffin‐embedded （FFPE） samples .Methods ：Total mR‐NA was isolated from 125 pairs of NSCLC FFPE tissues and their adjacent noncancerous samples .TGR7 expression was detected by using real‐time quantitative reverse transcription polymerase chain reaction （RT‐qPCR） .The correla‐tion between TGR7 expression levels and clinicopathological parameters was investigated .Results ：Expression of TGR7 mRNA in NSCLC tissues （1 .068 2 ± 0 .609 6） was significantly higher than that in adjacent tissues （0 .399 4 ± 0 .283 8） ,P〈 0 .001 .TGR7 mRNA level in the group with lymph node metastasis （1 .201 0 ± 0 .633 4） was significant‐ly higher than that without lymph node metastasis （0 .904 5 ± 0 .540 6） ,P = 0 .006 .TGR7 mRNA was remarkably higher in the group with the tumor diameter ≥ 3cm （1 .193 5 ± 0 .589 5） than that of tumor diameter〈 3cm （0 .932 3 ± 0 .606 6） ,P= 0 .016 ,and higher in the group of TNM stage Ⅲ ,Ⅳ （1 .176 2 ± 0 .632 9） than that of stage Ⅰ ,Ⅱ（0 .926 1 ± 0 .551 5） ,P= 0 .022 .Conclusion ：High expression of TGR7 mRNA plays an essential role in carcinogenesis and progression of NSCLC .TGR7 is expected to be an oncogene and also a biomarker in diagnosis ,prognosis and tar‐geted therapy of NSCLC .