高效液相色谱法测定乳制品中的呕吐毒素

Determination of Vomitoxin in Dairy Products by High Performance Liquid Chromatography

目的：建立乳制品中呕吐毒素的高效液相色谱法检测的方法.方法：样品经沉淀、过滤,上清液通过免疫亲和柱净化、浓缩,C18色谱柱分离,紫外检测器检测,标准曲线法定量.结果：该方法具有良好的准确度和精密度,在乳制品中添加回收率96％～106％,相对标准偏差0.45％～1.21％,检出限0.05 mg/kg.

A method for the determination of vomitoxin in milk powder by high performance liquid chromatography （HPLC） was developed. Samples were precipitated and filtered, and the supernatant was then purified and concentrated by immunoaffinity column chromatography, separated by C18 column chromatography, detected by ultraviolet detector and quantified by calibration curve method. The method proved to have good accuracy and precision. The spiked recovery of vomitoxin in dairy products was 98%-106%, and the relative standard deviations were 0.45%-1.21%. The detection limit of vomitoxin was 0.05 mg/kg.