麻栎树皮多酚含量的测定及抗氧化活性的研究

Polyphenol content and antioxidant activity of the barkof Quercus acutissima Carruth

采用分光光度法、DPPH法和连苯三酚红(PR)法对麻栎树皮提取物进行多酚化合物含量及抗氧化活性测定,讨论了用不同溶剂提取对多酚含量的影响.结果表明采用70%乙醇进行提取,提取物中多酚含量最高,为(6 589.91±43.32)mg/100 g.抗氧化活性测试中,麻栎树皮的不同溶剂提取物对DPPH自由基和羟自由基都表现出较高的清除率,其中麻栎树皮70%丙酮提取物的活性最好,在0.18 mg/m L质量浓度下,对DPPH自由基清除率为70%,在0.38 mg/m L质量浓度下,对羟自由基氧化率为75%.

Polyphenol content of the extracts from the bark of Quercus acutissima Carruth was determined by spectrophotometric method.The antioxidant activity of the extracts was evaluated by the DPPH free radical scavenging assay and pyrogallol autoxidation assay,respectively.Furthermore,the polyphenol contents of different extracts from this plant were investigated.The results show that the polyphenol content of 70% ethanol extract was the highest,which was（6 589.91 ± 43.32）mg/100 g.All of the different extracts from Q.acutissima have good activities against DPPH free radical and hydroxyl free radical,the results show that the 70% acetone extracts have exhibited better antioxidant activity against DPPH free radical at the concentration of 0.18 mg/mL with the inhibition rate of 70%,and 75% against hydroxyl free radical at 0.38 mg/mL.