胶原纤维吸附剂对单糖基黄酮苷类化合物的层析分离性能

Chromatographic Separation of Single Glucosyl Flavonoid Glycosides Using Collagenous Fiber as Adsorbent

以戊二醛交联胶原纤维吸附剂(CFA)为填料,对两种结构相近的单糖基黄酮苷类化合物(染料木苷和黄芩苷)进行了柱层析分离。结果表明,通过改变乙醇水溶液的浓度,可以调节CFA对染料木苷和黄芩苷的吸附选择性;当CFA的用量为6 g,层析柱的高径比为10∶1时,采用100%、90%和70%乙醇水溶液进行分步洗脱,染料木苷和黄芩苷能够得到分离。分离所得染料木苷和黄芩苷的纯度分别为98%和97%,回收率分别为99.35%和96.52%。

The collagenous fiber cross-linked by glutaraldehyde was used as an adsorbent （ CFA） for chromatographic separation of single glucosyl flavonoid glycosides. Genistin and baicalin with similar molecular structure were chosen as the representatives of flavonoid glycosides in the experiments is this paper. It was found that the adsorptive affinity and selectivity of CFA to genistin and baicalin could be adjusted by ethanol concentration in aqueous solution. In column chromatographic separation, where the CFA dosage was 6 g and the ratio of column height to diameter was 10∶1, genistin and baicalin were well separated by stepwise elution of 100 %-90 %-70 % ethanol aqueous solutions. The purities of the obtained genistin and baicalin were 98 % and 97 %, and their recovery rates were 99. 35% and 96. 52 %, respectively.