银杏LCC制备多孔生物载体及在人体肝细胞培养中的应用

Preparation of Porous Biological Carrier with Gingko LCC and Application in Culture of Human Hepatocytes

以银杏木质素-碳水化合物复合体(LCC)为原料,通过洗脱支架的方法制备医用多孔生物载体,并利用红外光谱、液相色谱、核磁共振波谱及扫描电子显微镜对该生物载体的主要成分含量、化学结构以及表面形态进行分析。结果表明:以银杏LCC为原料可以制备性能优良的生物载体。该载体不仅含有愈创木基型苯丙烷结构单元,而且糖组分中对人体肝细胞有较高生物相容性的半乳糖和甘露糖分别占3.30%和21.94%。制备的多孔生物载体孔隙率可达60%以上,平均孔径约为130μm。用多孔生物载体进行人体肝细胞培养,发现人体肝细胞能够成功地在银杏LCC多孔生物载体上生长,且细胞增殖较快,白蛋白分泌和葡萄糖代谢活性的测定结果表明肝细胞具有较高的代谢活性。研究结果表明银杏LCC多孔生物载体具有良好的生物相容性。

A porous biological carrier was prepared by the method of eluting stent with lignin-carbohydrate complexes （ LCC ） isolated from ginkgo wood. Fourier transform infrared spectrometer（FT-IR）, high performance liquid chromatograph（HPLC）, nuclear magnetic resonance （ NMR ） spectroscopy and scanning electron microscope （ SEM ） were applied to elucidate the composition, chemical structure and morphology of the biological carrier. The results indicate that a biological carrier with excellent performance can be prepared with gingko LCC as raw material. Not only the guaiacyl lignin subunits existe in this biological carrier, but also the galactose and mannose with high biological compatibility to human hepatocytes are found to be 3. 30% and 21. 94%, respectively. The porosity of prepared porous scaffold is more than 60% with average pore size of 130μm. The carriers were used to culture human hepatocytes. The results show that human hepatocytes can grow successfully on carriers. Furthermore, the human hepatocytes on carriers proliferate rapidly and show a high metabolic activity by determination of human albumin and glucose metabolism. These interesting results indicate that porous biological carrier prepared with gingko LCC have an excellent biocompatibility.