杜仲总木脂素及松脂醇二葡萄糖苷的纯化研究

Purification of Lignans and Pinoresinol Diglucoside from Eucommia ulmoides Oliv.

通过大孔吸附树脂-C18反相硅胶柱层析-正相硅胶柱层析联用技术,对杜仲总木脂素及松脂醇二葡萄糖苷(PDG)进行纯化研究。结果表明,含总木脂素23.33%和松脂醇二葡萄糖苷粗品0.960 5%的杜仲提取液经AB-8树脂一次纯化,可以制得71.61%的杜仲总木脂素有效部位以及8.070%的松脂醇二葡萄糖苷粗品,再经C18反相硅胶柱层析纯化,得到53.12%的松脂醇二葡萄糖苷。最后通过正相硅胶柱层析进一步纯化,得到91.50%的松脂醇二葡萄糖苷产品。

Purification of lignans and pinoresinol diglucoside （ PDG ） from Eucommia bark extract was studied by a combined method of macroporous adsorption resin-C18 column chromatography and silica gel column chromatography. The results indicated that AB-8 resin was the best one on the adsorption and desorption rates by comparing with the other 3 kinds of resins. The purity of effective part of lignans and the crude product of PDG were respectively from 23. 33% to 71. 61% and 0. 960 5% to 8. 070 %through AB-8 resin once. Then the purity of PDG increased to 53. 12 % after C18 purification and finally reached 91. 50 % after purifying by normal phase silica gel column chromatography.