吴茱萸致小鼠肝毒性分子机制研究

Study on molecular mechanism of Euodiae Fructus on liver toxicity in MICE

目的:研究吴茱萸提取物致小鼠肝毒性的分子机制。方法:30只KM小鼠随机分为3组,连续灌胃给予吴茱萸提取物15 d后,Western blot法检测小鼠肝脏Erk1/2,CDK8,CK1e,Stat3,Src蛋白表达量。结果:吴茱萸提取物导致Erk1/2,CDK8,CK1e表达量上调(P<0.01),Stat3,Src表达量下调(P<0.01)。结论:吴茱萸致肝毒性分子机制可能与Erk1/2,CDK8,CK1e,Stat3和Src信号分子相关。

Objective： To study the molecular mechanism of extracts from Euodia rutaecarpa on hepatotoxicity in mice. Method： Totally 30 KM mice were divided into 3 groups and orally administrated extracts from E. rutaecarpa for consecutively 15 days. The expressions of Erk1/2, CDKS, CK1e, Star3 and Src were detected by Western blotting method. Result： The extracts from E. rutaecarpa could up-regulated Erk1/2, CDK8 and CKle expressions （P 〈0.01 ） and down-regulate Stat3 and Src （P 〈0. 01 ）. Conclusion： The molecular mechanism of E. rutaeearpa on hepatotoxicity may be correlated with Erk1/2, CDK8, CKle, Stat3 and Src signal molecules.