花生秧部分替代马尾藻对仿刺参生长、消化的影响

Effect of Partial Replacement of Sea Weed Sargassum pallidum Meal by Peanut Vine Powder on Growth and Digestibility in Juvenile Sea Cucumber Apostichopus japonicus

利用花生秧粉替代配合饲料中的马尾藻粉,研究不同比例花生秧替代马尾藻的配合饲料对仿刺参幼参的生长和消化的影响。试验设6组,替代比例分别为0%(对照组)、8%、16%、24%、32%和40%,每组3个重复,试验进行60d。试验结果表明,各组仿刺参存活率均超过95%,且组间差异不显著(P>0.05);24%替代组仿刺参特定生长率最大(0.58%/d),高于对照组28.9%,8%替代组和16%替代组生长稍慢于对照组,但差异不显著(P>0.05),而32%替代组和40%替代组仿刺参生长明显慢于对照组(P<0.05);各替代组水体化学需氧量、氨氮和亚硝酸盐氮含量与对照组差异不显著(P>0.05);各替代组幼参对饲料消化率随替代比例变大而降低;试验前30d肠道内蛋白酶和淀粉酶活性均高于后30d,淀粉酶活性对照组表现最大,并有随花生秧粉替代比例变大含量下降的趋势;5个替代组仿刺参体腔超氧化物歧化酶和碱性磷酸酶活力与对照组差异不显著(P>0.05)。

The effects of partial replacement of sea weed Sargassum pallidum meal by peanut vine powder on growth and digestibility were studied in juvenile sea cucumber Apostichopus japonicus .The sea cucum‐ber juveniles w ere fed the diets containing the sea w eed meal w hich w as replaced by peanut vine pow der at 0% （control group） ,8% ,16% ,24% ,32% and 40% with triplication for 60 days .The results showed that there was survival rate of over 95% in all groups ,without significant difference （P〉0 .05） .It was found that the sea cucumber in group 24% replacement had the maximal specific grow th rate （0 .58% /d） , 28 .9% higher than that in the control group .There was poor growth in the juveniles in group 8% replace‐ment and group 16% replacement than than in the control group without significant difference （ P 〉0 .05） ,significantly lower in group 32% replacement and group 40% replacement that in the control group （P 〈 0 .05） .There were no significant differences in concentrations of COD ,ammonia‐N and NO2 -N in the water between the replacement groups （P〉0 .05） and the control group .The digestibility was found to be decreased in the juveniles fed the diets containing more peanut vine pow der ,higher protease and am‐ylase activities in alimentary canal in the early 30 days than in the later 30 days .The maximal protease ac‐tivity was observed in the juveniles in the control group ,being lower with the larger replacement ratio . There were no significant differences in activities of superoxide dismutase and alkine phosphatase in the coelomic fluid between the replacement groups and the control group （P〉0 .05） .