饵料中添加弧菌BC232对幼参免疫反应及抗病力的影响

Immune Responses and Disease Resistance of Juvenile Sea Cucumber Apostichopus japonicus Induced by Vibrio sp.BC 232

将弧菌BC232以105、107cfu/g和109cfu/g添加到基础饵料中,投喂仿刺参幼参,每一剂量组均设3个平行,对照组投喂基础饵料,试验在12个盛有100L过滤海水的塑料桶中进行,每桶放50头幼参,饲养试验进行30d和45d。试验结束后分析弧菌BC232对幼参免疫反应和抗病力的影响。试验结果表明,投喂弧菌BC232(107cfu/g和109cfu/g)45d幼参比对照组有较高的体腔细胞吞噬活力(P<0.01)。投喂弧菌BC232菌株105、107cfu/g和109cfu/g 30d及109cfu/g 45d幼参体腔液的溶菌酶活力分别显著高于对照组(P<0.05)。与对照组比较,投喂弧菌BC232菌株105cfu/g 30d和105、107、109cfu/g 45d幼参体腔液中的酚氧化酶极显著提高(P<0.01)。幼参投喂弧菌BC232菌株45d后用病原菌灿烂弧菌NB13菌株攻毒,各试验组幼参的发病率和死亡率明显低于对照组。本研究表明饵料中添加弧菌BC232可刺激幼参免疫反应,并提高其抗病力。

Vibrio sp .BC232 was added to the diets at 105 ,107and 109cfu/g .Juvenile sea cucumber A pos‐tichopus j aponicus was randomly distributed into 12 plastic tanks （100 L） at a density of 50 individuals per tank and fed one of the diets or basal diet （control） for 30 days and 45 days .Effects of V ibrio sp .BC232 on immune response and disease resistance against V .splendidus infection in sea cucumbers were analyzed after feeding experiment .Results showed that the phagocytic activity in coelomocytes of sea cucumbers fed Vibrio sp .BC232 at 107and 109cfu/g for 45 days was signicantly higher than that in the control group （P〈 0 .01） .The coelomic fluid lysozyme activities were signicantly enhanced in sea cucumbers receiving Vib‐rio sp .BC232 at 105 ,107 and 109 cfu/g for 30 days ,and 109 cfu/g for 45 days ,respectively ,compared to the control group （P 〈 0 .05） .Sea cucumbers fed V ibrio sp .BC232 at 105 cfu/g for 30 days ,and 105 ,107 and 109 cfu/g for 45 days ,respectively ,had higher coelomic fluid phenoloxidase activities than the animals fed basal diet（P 〈 0 .01） .Additionally ,10 sea cucumber from each group and control group were chal‐lenged with V .splendidus NB13 after feeding for 45 days .Cumulative incidence and mortality of sea cu‐cumber fed Vibrio sp .BC232 were found to be lower than those in the control group .The results showed that diet supplemented with V ibrio sp .BC232 stimulated the immune system of juvenile thus enhancing their resistance against V .sp lendidus .