热带芽孢杆菌的筛选及对人工废水效果研究

Screening and Purification of Bacillnsin Tropical Area and Effect on Artificial Wastewater

自海南热带海水养殖系统的底泥中筛选得到1株对人工废水净化效果明显的菌株LS-1305,通过对菌落形态、16SrDNA、生理生化试验,鉴定该菌株为弯曲芽孢杆菌。研究了弯曲芽孢杆菌LS-1305在人工废水中的生长特性及对凡纳滨对虾的安全性试验,并将密度为(2.5±0.3)×105cfu/mL的弯曲芽孢杆菌LS-1305活菌接种至化学需氧量、氨氮、亚硝酸盐初始质量浓度分别为(721.5±1.8)mg/L、(67.33±0.58)mg/L、68.56±2.08)mg/L的人工废水中,不间断充无菌空气培养48h。最终建立了该菌株在人工废水中随时间的生长关系。试验结果表明,该菌株对凡纳滨对虾安全,该菌株对人工废水的化学需氧量、氨氮、亚硝酸盐的去除率分别为91.61%、86.21%、87.22%。弯曲芽孢杆菌LS-1305具有显著改良海水养殖水体的潜在应用前景,为今后开发适合海南地区海水养殖环境的热带芽孢杆菌微生物制剂奠定了重要的基础。

A strain of Bacillus known as LS‐1305 was screened out from sediments of tropical marine aqua‐culture in Hainan province and was found to purify effectively artificial wastewater .Bacillus LS‐1305 was identified as B . f lexus by colonial morphology ,16S rDNA and biochemical characteristics tests .The LS‐1305 was inoculated at initial concentration of （2 .5 ± 0 .3）×10^5cfu/mL into the artificial wastewater with initial chemical oxygen demand of （721 .5 ± 1 .8） mg/L ,ammonia nitrogen （67 .33 ± 0 .58） mg/L ,and ni‐trite （68 .56 ± 2 .08） mg/L and aerated by sterile air for 48 h to investigate the growth characteristics of LS‐1305 and safety for Pacific white leg shrimp Litopenaeus vannamei .The growth regime of LS‐1305 with time in artificial wastewater was established and the LS‐1305 was approved to be security for Pacific white leg shrimp ,with the removal efficiency of 91 .61% for chemical oxygen demand ,86 .21% for ammo‐nia nitrogen and 87 .22% for nitrite ,indicating that LS‐1305 as a microbial preparations has potential for improvement of marine aquaculture water quality and environment in Hainan .