通过动态光散射分析血红蛋白A_2现象的分子机制

Analysis of the Molecular Mechanism of Hemoglobin A_2 Phenomenon by Dynamic Light Scattering

目的:通过动态光散射分析血红蛋白A2现象的分子机理。方法:首先,将正常成人新鲜抗凝全血制备成红细胞悬液和溶血液,利用淀粉-琼脂糖混合凝胶电泳分别分离得到红细胞和溶血液的Hb A和Hb A2,从凝胶中将这些区带分别切出,通过反复冻融,分离出区带内所含蛋白。用动态光散射方法对红细胞和溶血液的Hb A和Hb A2的粒子直径(粒径)进行分析。结果:红细胞Hb A的粒径与溶血液Hb A的粒径基本相同,含量最多的粒径约为7.5 nm左右;Hb A2的粒径大于Hb A,而且红细胞Hb A2的粒径(28.21 nm)明显大于溶血液Hb A2的粒径(13.54 nm)。结论:红细胞中的Hb A2并不是单独存在的,而是与某些成分结合形成复合体。

Objectives To analyze the molecular mechanism of hemoglobin A 2 （HbA2 ） phenomenon by dynamic light scattering （DLS） .Methods First ,prepare RBC suspension and hemolysate from fresh normal aldult whole blood .Then erythrocyte suspension and hemolysate was separated by starch -agarose mixed gel electrophoresis ,Finaly , HbA and HbA2 were acquired from the gel by freeze thawing method ,and then the size of these Hb was analyzed by DLS .Results The size of RBC HbA was the same with that of hemolysate＆#39;s ,and the diameter of most of the HbA was a_bout 7 .5 nm .The size of HbA2 is larger than that of HbA ,and the size of RBC HbA2 （28 .21 nm） was much larger than that of hemolysate HbA2 （13 .54 nm） .Conclusions HbA2 is not existed alone in RBC ,but in combination with the other components .