两肾一夹型高血压合并2型糖尿病大鼠模型的实验研究

Experimental Study on the Rat Models of Two-kidney and One-clip Hypertension Combined with Type 2 Diabetes

目的:建立肾血管性高血压合并2型糖尿病大鼠复合模型,通过检测不同时段的血压、血糖来观察此模型的稳定性。方法:雄性SD大鼠先采用两肾一夹法(2K1C)并饮用1.5%盐水建立高血压大鼠模型,在此模型成模的基础上喂养高糖、高脂饮食4周后经腹腔注射链尿佐菌素(35 mg/kg),然后继续予高糖、高脂饮食饲养至实验结束,检测不同时间点血压、空腹血糖、空腹胰岛素、血脂、血清C-反应蛋白,计算胰岛素敏感指数,并观察一般情况对所建模型进行评价。结果:高血压合并糖尿病组大鼠成模率70%,其血压、血糖值达到成模标准,至实验结束时较稳定,分别与假手术组、空白对照组比较差异有统计学意义(P<0.05)。结论:成功复制高血压合并2型糖尿病大鼠模型,该模型经济、操作简单、成模率高、稳定性好。

Objective ：Establishing a compound model with renal vascular hypertension combined type 2 diabetic rats so as to observe its stability by monitoring the blood and pressure in different time periods .Methods ：First ,we selected Male SD rats and adopted two kidney one clip method and drinking 1 .5% saline to establish hypertensive rat model ,on the basis of this model ,at the 4th week in high glucose ,and high fat feeding ,we injected abdominal cavity with strepto_zotocin （STZ 35mg/kg） .Second ,we continued to feed it with high -sugar high-fat diet until the experiment ended .Fi_nally ,we observed the changes of blood pressure ,fasting blood glucose（FBG） ,fasting insulin （FINS） ,lipids ,serum C- reactive protein （CRP） ,insulin sensitivity index （ISI） in different time points .Meanwhile ,we observed the model e_valuation in general situation .Results ：About 70% of renal vascular hypertension combined type 2 diabetic rats were made into models ,all the succeed models had a stable blood pressure and blood glucose from its established to the end of the experiment .It has a statistically significant difference compared with the control group ,sham operation group（P 〈0 .05） .Conclusion ：Successfully replicated hypertension combined with type 2 diabetic,which are economical, easy tooperate, and stable, can be made with a high success rate.