维吾尔族饲鸽者肺患者细胞因子水平变化及临床意义研究

Cytokines Level in Uygur Pigeon Breeder's Lung Disease Patients

目的探讨维吾尔族饲鸽者肺(PBL)患者支气管肺泡灌洗液(BALF)与血清中白介素(IL)-2、IL-10、肿瘤坏死因子-α(TNF-α)水平的变化及其在PBL发病机制中的作用。方法选取2012—2013年在新疆(喀什、和田)地区至少饲养鸽子1年以上的维吾尔族受试者,将新疆维吾尔自治区人民医院呼吸科确诊为PBL的受试者65例作为病例组,未患PBL的受试者120例作为阴性对照组;选取同期体检健康的未饲鸽的维吾尔族受试者120例为正常对照组。采用酶联免疫吸附试验(ELISA)检测3组受试者BALF与血清中IL-2、IL-10、TNF-α水平。结果病例组BALF与血清中IL-2、TNF-α的表达水平均较阴性对照组、正常对照组升高,阴性对照组较正常对照组亦升高(P<0.05)。病例组、阴性对照组BALF与血清中IL-10的表达水平较正常对照组均降低(P<0.05),而病例组与阴性对照组间差异无统计学意义(P>0.05)。结论维吾尔族PBL患者BALF与血清中IL-10水平降低,IL-2、TNF-α水平升高;IL-2、TNF-α可能参与了PBL的发生发展。

Objective To explore the pathogensis of pigeon- breeder- lung- disease（ PBL） by determining the changes of bronchoalveolar lavage fluid（ BALF）,serum interleukin（ IL）- 2,IL- 10,tumor necrosis factor（ TNF- α）.Methods From 2012 to 2013,65 Uygur pigeon- feeders diagnosed as PBL（ study group） by department of respiration,the Xinjiang Uygur Autonomous Region People＇s Hospital,120 healthy pigeon- feeders without PBL（ negative control group）,120 healthy subjects who did not feed pigeons（ normal control group） were enrolled in this study. BALF and the levels of IL- 2,IL- 10,TNF- α were detected by enzyme linked immunosorbent assay（ ELISA）. Results In the BALF and serum,IL- 2,TNF- α were higher in study group than in groups negative control,normal control（ P〈 0. 05）,higher in group negative control than in normal control（ P〈 0. 05）. And IL- 10 levels were lower in groups study,negative control than in normal control group（ P〈 0. 05）,there was no significant difference between groups study,negative control（ P〉 0. 05）. Conclusion In Uygur PBL patients,the BALF and serum IL- 10 decreases,IL- 2,TNF- α increase. IL- 2,TNF- α may be involved in the occurrence and development of PBL.