CD64对白血病合并细菌感染的早期诊断价值

Value of CD64 in Early Diagnosis of Leukemia Complicated with Bacterial Infection

目的探讨CD64对白血病合并细菌感染的早期诊断价值。方法选取白血病合并细菌感染患者50例、白血病非细菌感染患者48例和体检正常者38例,静脉抽取外周血,分别用流式细胞术检测CD64的表达及CD4/CD8比值,免疫比浊法检测降钙素原(PCT)和C-反应蛋白(CRP),以及电阻抗法检测中性粒细胞百分比(NEU%)。结果 1白血病合并细菌感染组CD64的表达显著高于白血病非细菌感染组和正常对照组,差异具有统计学意义(P<0.01)。2白血病合并细菌感染组的淋巴细胞CD4/CD8值要显著低于白血病非细菌感染组和正常对照组,差异具有统计学意义(P<0.01),提示其细胞免疫功能受到抑制。3从ROC曲线可以得出,PCT作为诊断早期感染的指标准确性优于CRP和NEU%,而CD64诊断早期合并细菌感染时的特异度和敏感度略高于PCT,所以CD64在诊断早期合并细菌感染时显示了更大的优势。结论 CD64对于白血病合并细菌感染具有良好的特异度,其对白血病合并细菌感染的早期诊断具有重要价值。

Objective To explore early diagnostic value of CD64 in leukemia complicated with bacterial infection.Methods Fifty leukemia patients complicated with bacterial infection ,48 leukemia patients without bacterial infection and 38 normal con‐trol group were enrolled into this study.Peripheral venous blood was collected.Expression of CD64 and the ratio of CD4/CD8 were detected by flow cytometry.Immunoturbidimetric assay was adopted to detect procalcitonin（PCT）and C‐reactive protein （CRP） ,and bioelectrlcal impedance analysis used to determine neutrophilic granulocyte percentage（NEU% ）.Results ①CD64 expression was significantly higher in the leukemia patients complicated with bacterial infection than in the other two groups（P〈0.01）. ②The ratio of CD4 to CD8 was significantly lower in the leukemia patients complicated with bacterial infection group than in the other two groups（P〈0.01）. ③The ROC curve showed that accuracy of PCT as an early diagnosis indicator for early infection was superior to CRP and NEU% ,and the specificity and sensitivity of CD64 in early diagnosis of leukemia combined with bacterial infection were higher than those of PCT.So CD64 had an advantage of early diagnosis in leukemia combined with bacterial infection.Conclusion CD64 has high specificity in diagnosis of bacterial infection ,and it played an important role in early diagnosis of leukemia complicated with bacterial infection.