产蛋鸡免疫程序对卵黄抗体效价的影响

Effect of Different Immune Procedures of Layer Chickens on the Yolk Antibody Titer

试验用小鹅瘟免疫原按8种免疫程序接种120日龄产蛋鸡,分别在接种后不同时间琼脂扩散试验试验检测卵黄抗体效价,筛选出最佳免疫接种程序。基础免疫,产蛋鸡每只肌肉注射小鹅瘟活疫苗1.0 m L(5羽份);在基础免疫后14 d进行第2次接种,加强免疫,产蛋鸡每只肌肉多点注射小鹅瘟灭活疫苗2.0 m L;在加强免疫后14 d进行第3次接种,强化免疫,产蛋鸡每只肌肉多点注射小鹅瘟灭活疫苗3.0 m L。

The experiment used eight different kind of immune procedures for 120 days layer chickens to immune Gosling Plague. In different time after inoculation, through agar diffusion reaction to detect valence of yolk anti-body, purpose was research the best immune procedure. The basic immune was 1.0mL Gosling Plague live vaccine （5 doses）for layer chickens by intramuscular injection. The second immune dose after the basic immune 14d, and the reinforced immune was 2.0mL Gosling Plague inactivated vaccine for layer chickens by multi-point intramus-cular injection. The third immune dose after the reinforced immune 14d,and the intensive immune was 3.0mL Gosling Plague inactivated vaccine for layer chickens by multi-point intramuscular injection.