烫伤致兔急性肺损伤早期肺泡灌洗液中促炎性细胞因子的变化

Effect of empyrosis on the proinflammatory cytokines of alveolar lavage fluid in rabbit with early acute lung injury

目的：探讨烫伤致兔急性肺损伤早期肺泡灌洗液中促炎性细胞因子的变化。方法：健康新西兰大白兔36只,随机分为6组,每组6只。其中1组为对照组（A组）,背部脱毛37℃水浸浴;另5组均为烫伤组（B组）,均建立30%总体表面积Ⅲ度烧伤模型,分别于烫伤后2、4、8、12、24 h时间点处死动物,作为B1、B2、B3、B4、B5组。观察各组肺组织病理,进行肺损伤评分,计算肺湿干质量比（W/D）,并应用双抗夹心的酶联免疫吸附法测定肺泡灌洗液中促炎性细胞因子TNF-α、IL-1β、IL-8的变化。结果：B组中所有兔子处死后经病理证实均达到30%总体表面积Ⅲ度烫伤。B1-B5组均可见到肺泡腔及间质内白细胞渗出、浸润,肺泡腔内见渗出液,肺泡隔增厚,肺泡及间质充血,部分肺泡塌陷、不张,透明膜形成,且随时程延长病变加重,肺损伤评分增加,B1-B5组与A组比较差异均有统计学意义（P〈0.05-P〈0.01）;B3-B5组肺W/D均较A组明显升高（P〈0.01）;B1-B4组肺泡灌洗液中TNF-α、IL-1β、IL-8水平较A组均升高（P〈0.05-P〈0.01）,其中TNF-α、IL-8均在12 h达到高峰（P〈0.01）,IL-1β在8 h达到高峰（P〈0.01）,而24 h时IL-8、IL-1β水平与A组比较差异均无统计学意义（P〉0.05）。结论：兔烫伤后早期,肺泡灌洗液中的促炎性细胞因子TNF-α、IL-1β、IL-8水平明显升高,与急性肺损伤的发生有关。

Objective： ： To investigate the effects of empyrosis on the proinflammatory cytokines of alveolar lavage fluid in rabbits with early acute lung injury. Methods： Thirty New Zealand white rabbits with 30% Ⅲ degree empyrosis model were established,and divided into five empyrosis groups（ n = 6） according to the sacrificing time of rabbits（ including postempyrosis 2,4,8,12,24 h time points）.The 6 healthy rabbits were burnt in water at 37 ℃ and served as the control group. The pathological changes of lung tissue,lung injury scores（ LIS） and Wet-dry weight ratio（ W / D） in all groups were observed. PMNs were isolated and purified in bronchoalveolar lavage fluid of rabbit. The proinflammatory cytokines（ TNF-α,IL-1β and IL-8） in the alveolar lavage fluid of each group were measured by ELISA. Results： The 30% Ⅲ degree empyrosis in all empyrosis groups were verified by pathology. The lyukopedesis and massive infiltration of PMN in alveolar spaces and interstitial edema,pereolate in alveolar spaces,alveolar septum thickerning,alveoli and interstitial hyperemia,alveolar collapse and atelectasis and transparent film formation were found,which aggravated with prolongation of time,and the lung injury scores increasing were measured in all empyrosis groups,the diffeences of which between all groups were statistically significant（ P〈0. 05 to P〈0. 01）. The W / D in rabbits at 8,12 and 24 h after empyrosis were significantly higher than that in control group（ P〈0. 01）. The levels of TNF-α,IL-1β and IL-8 in the alveolar lavage fluid after 2,4,8,12 h of empyrosis were higher than those in control group（ P〈0. 05 to P〈0. 01）. The levels of TNF-α and IL-8 peaked at 12 h after empyrosis（ P〈0. 01）,the level of IL-1β peaked at 8 h after empyrosis（ P〈0. 01）,and the differences of the levels of IL-8 and IL-1β between at 24 h after empyrosis and control group were not statistically significant（ P〉0. 05）. Conclusions： The levels of TNF-α,IL-1β and IL-8 in the alveolar lavage fluid significantly increase in early acute lung injury of rabbits with empyrosis,which may lead to the occurrence of early acute lung.