2种方法处理精液液化异常的比较分析

Comparison of two methods of handling non-liquefaction semen

目的:比较机械混匀法和菠萝蛋白酶消化法处理液化异常精液的质量。方法:选取60份经常规分析证实为精液液化异常标本,标本量均≥1.5 ml,每份标本均分为3组:A组即对照组,未经任何处理;B组,采用机械混匀法处理;C组,采用菠萝蛋白酶消化法处理。首先应用西班牙人类精液分析微机辅助智能系统分别对3组样本的精子浓度、精子活力等参数进行检测;同时采用伊红染色法检测精子存活率;改良巴氏染色法分析精子正常形态率。结果:3组精子浓度和存活率差异均无统计学意义(P>0.05);B组和C组精液液化时间均比A组明显缩短,精子活力也显著高于A组(P<0.01);B组精子正常形态率明显低于A组与C组(P<0.01);C组精液液化时间明显短于B组(P<0.01)。结论:菠萝蛋白酶消化法比机械混匀法更适于处理精液液化异常,可明显缩短精液常规分析时间,降低精液黏度,避免取样误差,提高检测的准确性。

Objective： To compare the methods of mechanical mixing and bromelain treatment in handling non-liquefaction semen.Methods： Sixty cases of non-liquefaction semen were taken from the patients in our department and all the sample＇ s volume was≥1. 5 ml after routine examination. Every sample was divided into group A（ control group）,group B（ semen handled by mechanical mixing） and group C（ semen treated with bromelain）. Human Computer-aided Semen Analysis System of Spain was used to detect the sperm density and motility; at the same time,Eosin Staining was used to detect the sperm viability,and Modified Papanicolaou Staining was employed to analyze the rate of the normal morphology sperm. Results： There was no significant difference among the three groups in the sperm density and viability（ P〉0. 05）. The time of semen liquefaction and sperm motility in group B and group C were both significantly higher than those in group A（ P〈0. 01）. However,the rate of normal morphology sperm in group B was significantly lower than that in group A and group C（ P〈0. 01）. The time of semen liquefaction in group C was significantly shorter than that in group B（ P〈0. 01）. Conclusions： Bromelain method is superior to mechanical mixing in handling abnormal liquefaction semen. It can not only shorten the time of semen routine analysis but reduce the semen viscosity,which will avoid sampling error.