水稻微核心种质中广亲和基因S5^n的筛选

Screening of Wide Compatibility Gene S5n in Mini-core Germplasm of Cultivated Rice

利用S5n的功能标记扩增197份栽培稻微核心种质,从中筛选携带广亲和基因S5n的材料,并检验亲和性。结果表明,共筛选到11份(湖恢628、本邦谷、毫补卡、小红谷、饭毫皮、飞蛾糯2、特青选恢、包协-7B、毫马克(K)、清可、老造谷)含有S5n特异带型的水稻种质,将其与籼稻9311和粳稻日本晴杂交,其F1结实率均高于75%,表现广亲和性。这批广亲和性材料可为籼粳亚种间的优势利用及品种选育提供更多的可利用资源。

In this study,197 mini-core germplasms of cultivated rice were screened using S5n function markers,1 1 genotypes with wide compatibility S5n gene were confirmed which were Huhui 628,Benbanggu,Haobuka,Xiaohonggu,Fanhaopi,Feienuo 2,Teqingxuanhui,Baoxie-7B, Haomake,Qingke and Laozaogu,and their compatibility were verified by crossing them with typical indica 9311 and japonica Nipponbare.The result indicated that the fertility of all the F1 was above 7 5%,presenting the wide compatibility.These wide compatibility materials provided more available resources for utilizing subspecies heterosis and breeding new varieties.