摘要
以籼稻Kra Dang Ngah成熟胚为材料,探讨了培养基、植物凝胶质量浓度、激素配比、碳水化合物等因素对其再生体系的影响。结果表明,籼稻Kra Dang Ngah胚性愈伤组织的最佳分化培养基为ARDA培养基,植物凝胶为3.0g/L,细胞分裂素(6-BA、KT)与生长素(NAA)配比为6∶1,碳水化合物为山梨醇15g/L、水解酪蛋白1g/L,在此条件下再生芽获得频率较高,为75.0%。将上述条件下获得的再生芽转移到生根培养基(MS培养基+0.1mg/L IAA+30g/L蔗糖)中可获得100%具有完全根系的再生植株,移栽炼苗7d后生长1个月,再生植株的成活率达到100%。
The embryogenic calli of indica rice Kra Dang Ngah was as material and the effect of culture media,phytagel concentrations,the ratio of plant phytohormones,carbohydrates on plant-let regeneration were studied.The results showed that the optimum culture media was ARDA,the optimum concentration of phytagel was 3.0 g/L,the optimum ratio of cytokinin(6 BA,KT)to auxin(NAA)was 6∶1 ,the optimum carbohydrates was 1 5 g/L sorbitol and 1 g/L casein hydrol-ysate.Under this conditions the highest regeneration shoots was obtained with 7 5 .0%.Then the regeneration shoots were transfered to root initiation media(MS+0.1 mg/L IAA+30 g/L su-gar),the 100% regeneration plantlets with complete roots were obtained.After seven days of accli-matization,the survival rate of plantlets was 100% after growth for one month.
出处
《河南农业科学》
CSCD
北大核心
2014年第11期27-31,共5页
Journal of Henan Agricultural Sciences
基金
宁夏自然科学基金项目(NZ14005)
关键词
籼稻Kra
Dang
Ngah
再生体系
影响因素
indica rice Kra Dang Ngah
plantlet regeneration system
regeneration frequency