籼稻Kra Dang Ngah植株再生体系影响因素研究

Study on Factors Influencing Plantlet Regeneration of Indica Rice(Oryza sativa L.) Kra Dang Ngah

以籼稻Kra Dang Ngah成熟胚为材料,探讨了培养基、植物凝胶质量浓度、激素配比、碳水化合物等因素对其再生体系的影响。结果表明,籼稻Kra Dang Ngah胚性愈伤组织的最佳分化培养基为ARDA培养基,植物凝胶为3.0g/L,细胞分裂素(6-BA、KT)与生长素(NAA)配比为6∶1,碳水化合物为山梨醇15g/L、水解酪蛋白1g/L,在此条件下再生芽获得频率较高,为75.0%。将上述条件下获得的再生芽转移到生根培养基(MS培养基+0.1mg/L IAA+30g/L蔗糖)中可获得100%具有完全根系的再生植株,移栽炼苗7d后生长1个月,再生植株的成活率达到100%。

The embryogenic calli of indica rice Kra Dang Ngah was as material and the effect of culture media,phytagel concentrations,the ratio of plant phytohormones,carbohydrates on plant-let regeneration were studied.The results showed that the optimum culture media was ARDA,the optimum concentration of phytagel was 3.0 g/L,the optimum ratio of cytokinin（6 BA,KT）to auxin（NAA）was 6∶1 ,the optimum carbohydrates was 1 5 g/L sorbitol and 1 g/L casein hydrol-ysate.Under this conditions the highest regeneration shoots was obtained with 7 5 .0%.Then the regeneration shoots were transfered to root initiation media（MS＋0.1 mg/L IAA＋30 g/L su-gar）,the 100% regeneration plantlets with complete roots were obtained.After seven days of accli-matization,the survival rate of plantlets was 100% after growth for one month.