南方红豆杉紫杉醇产生菌的分离与鉴定

Isolation and Identification of Endophytic Fungus Producing Paclitaxel from Taxus chinensis var.mairei

从南方红豆杉(Taxus chinensis var.mairei)根部树皮内表皮中分离得到69株内生真菌,通过HPLC检测发酵液中紫杉醇含量,共获得7种紫杉醇产生菌,同时,通过形态学分析以及ITS序列分析确定分别属于链格孢属(Alternaria)、葡萄孢盘菌属(Botryotinia sp.)、刺盘孢属(Colletotrichum)、拟茎点霉属(Phomopsis)和枝孢菌(Cladosporium),其中链格孢属真菌紫杉醇产量最高,平均为271.54μg/L,是目前见诸报道的产量最高的链格孢菌,亦是从红豆杉根部树皮中筛选出的紫杉醇产量较高的野生菌株之一。该菌株的发现为紫杉醇高产菌的选育提供了又一优良的种质资源。

69 strains of endophytes were isolated from root skin of Taxus chinensis var.mairei,which were belonged to 16genera（groups） according to their morphological character.HPLC method was used to detect the concentration of paclitaxel in ferment.7 strains were detected with the ability to synthesize paclitaxel.Combined with ITS sequence analysis,these 7strains were identified as Alternaria、Botryotinia、Colletotrichum、Phomopsis and Cladosporium sp.The Alternaria sp.had the highest paclitaxel productivity,with the average up to 271.54 μg/L.It will provide another resource for selecting of high-yield strain of paclitaxel.