HPLC测定羌活药材中羌活醇和异欧前胡素含量的效果分析

Effectiveness analysis of determining the content of notopterol and isoimperatorin in notopterygium

目的研究高效液相色谱（HPLC）测定不同产地和不同采收期羌活药材中羌活醇和异欧前胡素含量的效果。方法采用HPLC仪,色谱条件：色谱柱采用Waters symmetry C18（4.6mm×250.0mm,5μm）,流动相为乙腈-水（45∶55）,流速为1mL/min,柱温30℃,检测波长为310nm。结果羌活醇和异欧前胡素的线性范围分别为0.240 8-1.505 0μg和0.117 6-0.735 0μg,平均加样回收率（n=6）分别为0.98%和1.42%。不同产地羌活药材中羌活醇和异欧前胡素含量差别较大,采收期和生长年限对这2种成分的含量均有影响。结论 HPLC测定简单快捷,为确定羌活药材的最佳产地及采收期提供了依据。

Objective To establish the effect of determining the content of notopterol and isoimperatorin in notopterygium from different habitat and harvest time by HPLC method.Methods HPLC instrument was used,Waters symmetry C18（4.6mm × 250 mm,5μm）was adopted with a mobile phase of acetonitrile-water（45∶55）,the separation was carried out at a flow rate of 1mL/min,column temperature was 30℃and the detection wavelength was 310 nm.Results Notopterol and isoimperatorin showed good linearity in the ranges of 0.240 8-1.505 0μg and 0.117 6-0.735 0μg with average recoveries rate（n=6）of 0.98% and 1.42%respectively.The content of notopterol and isoimperatorin in crude drugs of notopterygium from different habitat marked difference,and harvest period and growth years made effect to content of two components.Conclusion HPLC method is easy and fast,and can be used as a basis for confirming the best habitat and harvest period of notopterygium.