洛阳市非EV71、非CA16肠道病毒中优势毒株状况分析

Status analysis of prevailing strains of non-EV71 and non-CA16 enteroviruse

目的了解洛阳市引起手足口病的普通肠道病毒的构成情况,警惕比例较高毒株发展为新的优势毒株,为洛阳市的手足口病防控提供科学依据。方法采用实时荧光PCR(RT-PCR)对洛阳市手足口病的送检粪便标本进行核酸鉴定,随机选取9例普通肠道病毒阳性,而柯萨奇病毒A16型(CA16)和新肠道病毒71型(EV71)阴性的标本,进行5′UTR测序,确定毒株的亚型。利用CA6的特异性引物,选取75例普通肠道病毒阳性标本(CA16和EV71阴性),用RT-PCR方法鉴定CA6的阳性数量,分析CA6的构成比。结果 9个普通肠道病毒5′UTR测序结果进行BLAST初筛,结果显示CA6为5株,在普通的肠道病毒中为优势毒株;75例普通肠道病毒中,共检测出CA6 20例。CA6在普通肠道病毒中的比例为29.76%(25/84);25例CA6中,男13例,女12例;引发重症的病例为2例;城市地区5例,乡村20例。结论洛阳市引起手足口病的普通肠道病毒的构成较为复杂,在引起手足口病的普通肠道病毒中CA6病毒所占比例较高,对此应引起重视。

Objective To investigatethe constituents of enteroviruse which can cause hand-foot-and-mouth disease（HFMD）,and prevent against the development of new prevailing strains and provide basis for prevention and control of HFMD in Luoyang city.Methods Reverse-transcription polymerase chain reaction（RT-PCR）was used to identify the type of RNA of stool specimen of HFMD cases in Luoyang,to distinguish the subtype of strain,9enterovirus-positive specimens which coxcackie virusA16（CA16）-and enterovirus71（EV71）-negative were selected randomly for 5′UTR sequencing.strain-specific primers were used to detect positive samples in 75CA16-and EV71-negative but other enterovirus-positive specimens by using real time RT-PCR.Composition characteristics of CA6 were further analyzed.Results Primary screening was conducted with the results of 5′UTR sequencing of 9commonent erovirus-positive specimens and there were 5CA6-positive,which were prevailing strains;there were 20CA6-positive of75commonenterovirus-positive specimens,the proportion of CA6-positive in enterovirus-positive specimens was 29.76%（25/84）.Thirteen samples were collected from male and 12 from female,2cases resulted in severe symptoms,5from city and 20 from country.Conclusion The composition of commonent erovirus which results in HFMD was complex and the proportion of CA6 was higher,which should be pay more attention.