湛江金银花内生真菌多样性研究

Diversity of endophytic fungi in Zhanjiang honeysuckle

为了解湛江金银花内生真菌的多样性,比较分析不同地区金银花真菌种类差异。采用真菌形态鉴定同时结合真菌ITS序列方法对金银花内生真菌的多样性进行研究。结果从湛江金银花分离鉴定了内生真菌17株,分别属于9个属17个种,其中拟盘多毛孢属Pestalotiopsis为优势属,占分离总株数的29.41%,叶部分离的主要是木霉属Trichoderma和拟茎点霉属Phomopsis,茎部分离的是镰刀菌属Fusarium,花部分离得到的主要是拟盘多毛孢属Pestalotiopsis。不同组织部位的湛江金银花内生真菌在数量和种群组成中存在差异,表现出较丰富的多样性。

To study the diversity of endophytic fungi in Zhanjiang honeysuckle,the difference of honeysuckle endophytic fungi in different regions was analyzed. The diversity of endophytic fungi was identified by fungal ITS sequences colony and morphology identification. 17 strains from Zhanjiang honeysuckle were divided into nine genera, 17 species. Pestalotiopsis was the dominant genus in 17 genera, accounting for 29.41% of the total, while the main part from leaves was Trichoderma and Phomopsls, the detach from stem was Fusarium, Peswlotiops was mainly separated from flower. Different tissues of Zhanjiang honey suckle endophyte showed differences in the number and species composition, showing rich diversity.