水稻株型相关性状的QTL分析

Analysis of QTLs for Plant Type Traits in Rice(Oryza sativa)

以南方籼型杂交稻恢复系泸恢99和北方粳型超级稻沈农265杂交衍生的重组自交系群体（recombinant inbred lines,RILs）为试验材料,对株型性状（株高、穗长、分蘖和叶片性状）进行不同环境下的数量性状基因位点（quantitative trait locus,QTL）分析。共检测到39个相关QTL,分布在水稻第1、第2、第3、第6、第7、第8和第9染色体上,LOD值介于2.50~16.90之间,有11个QTL能在两年中被检测到。株型相关的QTL在染色体上成簇分布,主要分布于第1、第6和第9染色体上,这可能与株型性状间显著或极显著相关有关。其中,在第9染色体上RM3700B–RM7424区间存在1个QTL簇,含4个QTL,即qPH9、qPL9、qFLL9和qSLL9,这4个QTL在2年中均被检测到。此外,进一步鉴定出5个能稳定表达的QTL,其中,qPH8、qFLW6和qSLW6效应较大。这些信息综合反映了株型相关性状遗传的复杂性,有助于更全面地了解和掌握株型性状的遗传基础。

A recombinant inbred lines （RILs） derived from the across between indica restorer line Luhui 99 and super japonica cultivar Shennong 265, was used to analyze the quantitative trait loci （QTLs） for plant height, panicle length, tillers, and leaf traits in 2012 and 2013. A total of 39 QTLs were detected on chromosomes 1, 2, 3, 6, 7, 8, and 9 with LOD score ranging from 2.50 to 16.90. Eleven of them were detected in both 2012 and 2013. Moreover, QTL clusters were detected on chromosomes 1, 6, and 9, which may be related to significant or highly significant correlations between plant type traits. Among them, the QTL cluster on chromosome 9 contained four QTLs, qPH9, qPL9, qFLL9, and qSLL9 in the interval between RM3700 and RM7424, and the four QTLs were detected in both years. In addition, five major QTLs were first reported, among which three QTLs （qPH8, qFLW6, and qSLW6） had the larger effect. The results facilitate further understanding of the genetic basis for plant height, panicle length, tillers and leaf traits.