VVC与RVVC患者治疗前后阴道菌群的对比研究

Comparative study of vaginal microflora of VVC and RVVC patients before and after treatment

目的从菌群总体角度分析和比较单纯性外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)患者与复发性外阴阴道假丝酵母菌病(recurrent vulvovaginal candidiasis,RVVC)患者临床治疗前后阴道菌群的差异,了解临床治疗与阴道微生态多样性的相关性。方法采集VVC患者(VVC组,3例)及RVVC患者(RVVC组,3例)女性阴道后穹窿分泌物,提取细菌总基因组DNA,扩增16s rRNA V4区基因,以得到样品的物种信息用于对比研究。结果 VVC组和RVVC组两组阴道菌群未见显著差异,而以个体为特点区分聚类。用药后,VVC组菌群的α-多样性未见明显改变,但菌群结构改变显著,体现在乳酸菌比例增加,加德纳菌和变异菌属比例降低。用药后RVVC组菌群的α-多样性和菌群构成均未见显著改变(P>0.05)。结论VVC组和RVVC组阴道菌群无显著差异。用药后,VVC组菌群结构改变较RVVC组显著。

Objective To analyze and compare differences of vaginal microflora of patients with VVC and RVVC and explore the correlation of clinical treatment and vaginal microflora diversity after clinical treatments .Methods Twelve vaginal swabs of the va-ginal fornix were collected from 3 VVC patients and 3 RVVC patients.Genomic DNA of the samples was extracted for amplifying the 16s rRNA V4 hypervariable region were employed to analyze the information of the species from the samples .Results The vaginal micro-flora were clustered by individual rather than VVC and RVVC .The VVC group exited no difference in alpha-diversity but significant structural change that charactered by increasing in the proportion of Lactobacillus and decreasing in Gardnerella and Atopobium after treatment.However,no significant differences in alpha-diversity and microbial structure exhibited in RVVC group （P〉0.05）.Conclu-sion The vaginal microflora existed no different structure between VVC group and RVVC group .Compared with RVVC group ,the VVC group demonstrated significantly microbial structure .