一点红HPLC指纹图谱研究及咖啡酰奎宁酸类成分的含量测定

Study on HPLC fingerprints of emilia sonchifolia and content determination of caffeoylquinic acids

目的:研究一点红的HPLC指纹图谱,并测定该药材中新绿原酸1、绿原酸2、隐绿原酸3这3个咖啡酰奎宁酸类的含量,为其质量控制提供参考。方法:采用Phenomenex C18色谱柱(250mm×4.6mm,4μm),0.1%甲酸乙腈-0.1%甲酸水为流动相,梯度洗脱,流速1.0m L/min,检测波长326nm,柱温为30℃。对10批不同产地的一点红药材的HPLC图谱进行相似度评价。采用外标法,利用1-3的混合对照品测定其在10批药材中的含量。结果:确定该10批不同产地的一点红药材具有18个共有峰,10批药材的相似度范围为0.851-0.992;测定了10批一点红中的3个咖啡酰奎宁酸类成分的含量,结果显示,不同产地一点红中3个成分的含量差异明显。结论:HPLC指纹图谱方法与3个成分的含量测定方法准确、稳定、简单,可为一点红质量控制提供参考。

Objective： To establish an HPLC method for fingerprint analysis of emilia sonchifolia and simultaneous determination of three caffeoylquinic acids （neochlorogenic acid（1）, chlorogenic acid（2） and cryptochlorogenin acid（3） so as to provide a scientific basis for the quality control. Methods： The separation of HPLC was performed on a Phenomenex C18 column （250mm×4.6mm, 4 μm） with 0.1% formic acid acetonitrile-0.1% formic acid as the mobile phase in a gradient elution at a flow rate of 1.0mL/min. The detection wavelength was set at 326nm, and the column temperature was set at 30℃. The similarity of 10 batches of emilia sonchifolia from different fields was appraised by the similarity evaluation system. By using the external standard method and mixture reference substance, their contents of the 10 batches of herb were determined. Results： 18 peaks were identified as the characteristic fingerprints of the 10 batches of emilia sonchifolia, and the similarity was from 0.851 to 0.992. The contents of neochlorogenic acid（1）, chlorogenic acid（2） and cryptochlorogenin acid（3） in emilia sonchifolia from different habitats were different from each other. Conclusion： The method is accurate, stable and simple, which could be used for quality control of emilia sonchifolia.