秦艽抗肝损伤活性部位筛选

Screening of active part of Gentiana macrophylla Pall. in the liver protection

目的:筛选秦艽发挥保肝作用的有效部位。方法:用系统溶剂法对秦艽95%乙醇提取物进行萃取分离,分成4个极性部位,用所得不同极性部位的药液灌胃大鼠7d后,皮下注射CCl4花生油溶液,致急性肝损伤大鼠模型,测定血清学指标(ALT,AST)并制作病理学切片,观察肝组织病理改变。结果:与模型组比较,秦艽提取物水溶性部位的高、低剂量组均可明显抑制大鼠血清中ALT、AST的升高(P<0.01);病理学切片显示,秦艽提取物水部位能明显减轻模型动物肝脏病变程度。结论:水溶性部位为秦艽保肝护肝的活性部位。

Objective： To screen the effective fraction with liver protection activity from Gentiana macrophylla Pail. Methods： Fractions of Gentiana macrophylla Pail. with different polarities were obtained from a serious of extraction solutions. The activities of AST and ALT were measured in liver injury mouse model induced by carbon tetrachloride （CCl4）. Results： With the system by method of solvent extraction separation of ethanol extract of Gentiana 95%, divided into 4 polar fractions, with the different polar parts of liquid gastric perfusion rat 7 days later, subcutaneous injection of CCl4 peanut oil solution, induced acute liver injury in a rat model, determination of serological indexes （ALT, AST） and the production of pathological sections, observation of pathological changes of liver tissue. Conclusion： Compared with the model group, Gentiana macrophylla extract water at high, low dose group can significantly inhibit ALT in rat serum, the increase of AST （P〈0.01）; pathological biopsy showed, Gentiana macrophylla extract water position can significantly reduce the severity of the liver lesions in animal model.