维生素E在大鼠肾脏缺血再灌注中的作用

Effect of vitamin E on renal suffering ischemia/reperfusion injury of rats model

目的 观察维生素E（VE）对大鼠肾脏缺血再灌注（I/R）的作用.方法 18只健康雄性Wistar大鼠随机分为假手术组、I/R组、VE＋ I/R组,每组6只.所有动物于I/R术24h后处死,取肾组织进行光镜检查,留取血清测定尿素氮（BUN）和肌酐（SCr）的浓度.Western印迹测定肿瘤坏死因子α（TNF-α）蛋白的表达.结果 HE、PAS染色结果显示,I/R组肾小管及间质病理损伤显著重于假手术组,VE＋ I/R组小管间质损伤程度较肾脏I/R组明显减轻.与假手术组相比,I/R组以及VE＋I/R组BUN和SCr均明显升高[BUN：（10.13±2.14）、（7.67±1.63）、（3.85±0.21） mmol/L,SCr：（80.33±7.15）、（63.67±5.40）、（48.67±3.61）μmol/L]（P均＜0.05）;VE＋ I/R组较I/R组BUN和SCr水平显著降低（P均＜0.05）.Western Blotting检测显示,VE＋ I/R组肾组织TNF-α蛋白表达与I/R组相比显著降低（P＜0.05）.结论 VE可能通过抑制TNF-α表达发挥对I/R中肾脏保护作用.

Objective To investigate the effects of vitamin E （VE） on renal ischemia/reperfusion （I/R） injury of rats.Methods A total of 18 male Wistar rats were randomly divided into sham-operated group,I/R group,VE ＋ I/R group,and each group of 6 rats.All the animals were killed at the end of 24 h of reperfusion.Nephridial tissue were examined by light microscopy,and the level of blood urea nitrogen（BUN） and serum creatinine （SCr） were measured.The protein expressions of tumor necrosis factor α （TNF-α） were detected by Western blotting.Results Compared with sham-operated group,tubulointerstitial pathological injury in I/R group was significantly aggravated,which was shown by HE and PAS stain.Compared with I/R group,the degree of morphological changes as well as renal dysfunction in VE ＋ I/R group were obviously lessened.Meanwhile,the levels of BUN,SCr in I/R group,VE ＋ I/R group were （10.13 ± 2.14） mmol/L and （7.67 ± 1.63） mmol/L,（80.33 ±7.15） μmol/L and （63.67 ±5.40） μ mol/L,significantly higher than those in shamed-operated group （（3.85 ± 0.21） mmol/L,（48.67 ± 3.61） μmol/L;P 〈 0.05）.And the level of BUN and SCr in VE ＋ I/R group were significant lower than those in I/R group（P 〈0.05）.Western Blotting showed that the protein expressions of TNF-α in VE ＋ I/R group were obviously lower compared with those in group of I/R without VE treatment （P 〈 0.05）.Conclusion Vitamin E can attenuate over-expressions of TNF-α in kidney following I/R,thus protect against structural damages and renal dysfunction in I/R rat models.