籼稻R996转Pi9基因纯系的筛选及稻瘟病抗性鉴定

Screening of homozygous Pi9 gene transgenic lines of Indica cultivar R996 and identification of its rice blast resistance

采用农杆菌介导法将抗稻瘟病Pi9基因导入籼稻品种R996,获得转基因植株,并对这些转基因植株进行遗传分析。结果表明:Pi9基因已经整合到受体细胞基因组中,并在转录水平上得到了有效表达;对转基因植株后代进行潮霉素抗性、GUS染色和PCR检测分析,鉴定出Pi9基因阳性转化子;经稻瘟病抗性鉴定,从T1代符合孟德尔分离比(1∶2∶1)的分离群体中,成功筛选出转Pi9基因的籼稻R996纯系。

Pi9, the first cloned gene with broad-spectrum resistance to rice blast, was introduced into Indica cultivar R996 via Agrobacterium-mediated transformation. The subsequent genetic analysis showed that Pi9 was successfully introduced into the genome of the receptors, which displayed stable expression of Pi9 at transcription level. The offspring of the transgenic plants were analyzed by hygromycin resistance assay, GUS staining and PCR detection. And the homozygous Pi9/R996 transgenic lines were identified from the T1 generation showing 1 ： 2 ： 1 Mendel segregation through rice blast resistance assay conducted on Pi9 positive transformants.