青海野生中国沙棘AQP基因的扩增及序列分析

Amplification and Sequence Analysis of AQP Gene in Hipphphae rhamnoides subsp.sinensis in Qinghai

以青海野生中国沙棘的嫩叶为材料,扩增其AQP基因并测序。结果表明,所得中国沙棘AQP基因的碱基数量为481个,其中A碱基103个,T碱基143个,G碱基126个,C碱基109个。序列分析表明,QP基因片段编码160个氨基酸,其中强碱性氨基酸有10个,强酸性氨基酸有7个,疏水性氨基酸有73个,亲水性氨基酸有31个。同源性比对表明,中国沙棘与杨、山核桃、栎树、葡萄等植物的AQP基因具有较高的同源性。为深入研究青海野生中国沙棘在干旱胁迫下的抗旱分子机制及沙棘抗旱分子改良积累了资料。

The fragment of AQPgene of wild Hippophae rhamnoides subsp.sinensis occurring in Qinghai Province was amplified by the specific primers and sequenced,then the nucleotide sequence and amino acid sequence of AQPgene was identified and summarized.The sequence analysis indicated that the total length of AQPgeng was 481 bp,and the nucleotide composition analysis showed that the number of A,T,G,C were 103,143,126 and 109,respectively.The analysis of the amino acid sequence of AQPgene indicated that the total length of amino acid of AQPgene was 160,and the animo acid composition analysis showed that there were 10 strongly basic amino acids,7strongly acidic amino acids,73 hydrophobic amino acids and 31 polar amino acids.This results would lay a foundation for the further study on the molecular mechanism of drought resistance of wild H.rhamnoides subsp.sinensisin Qinghai under drought stress and accumulate the information for the improvement of drought resistance of H.rhamnoides.