miR-30c对前列腺癌的功能调控作用

The function of miR-30c which may regulate prostate cancer progression

目的研究mi R-30c在前列腺癌中的功能调控作用,获得其调控前列腺癌侵袭及转移的可靠证据,进而揭示其在前列腺癌中可能的调控机制。方法从mi RNA芯片检测结果中获得前列腺癌相关的差异表达的mi R-30c分子,进一步通过mi R-30c过表达质粒转染前列腺癌细胞,运用Transwell检测细胞侵袭变化,划痕实验检测细胞转移变化。结果 LNCa P/DU145转染质粒后,mi R-30c的表达水平显著高于阴性对照组(LNCa P:倍数=3.87,P<0.001;Du145:倍数=4.32,P<0.001)。Transwell侵袭实验表明,mi R-30c转染组的LNCa P/DU145细胞侵袭的数量显著低于对照组(LNCa P:67 vs.120个/视野,P<0.001;DU145:130 vs.220个/视野,P<0.001)。划痕实验结果发现mi R-30c转染后,LNCa P/DU145细胞实验组转移的数量显著低于对照组(LNCa P:241 vs.520个/视野,P<0.001;DU145:490 vs.660个/视野,P<0.001)。结论 mi R-30c可抑制前列腺癌细胞的侵袭及转移,这说明mi R-30c在前列腺中确实起着抑癌的作用,其可能通过KRAS-MAPK信号通路抑制前列腺癌的侵袭及转移。

ObjectiveTo study the function of miR-30c which may regulate prostate cancer progression, and we get the reliable evidence that miR-30c regulates the invasion and metastasis of prostate cancer, reveals the possible regulatory mechanism in prostate cancer.MethodsWe chose miR-30c from the differentially expressed miRNAs through miRNA microarray on the human samples. Further by miR-30c overexpression plasmid transfecting prostate cancer cells, using Transwell test cell invasion changed, wound healing test cells shift change.ResultsThe miR-30c expression levels were measured at 48 hours after transfection by qRT-PCR. Data showed that miR-30c expression level in miR-30c-transfected cells was significantly increased compared that in control cells （LNCaP, fold=3.87, P〈0.01; Du145, fold=4.32,P〈0.01）. The results of Transwell assay clearly revealed that overexpression of miR-30c significantly reduced the migrated miR-30c-transfected LNCaP and DU145 cells compared with control cells （LNCaP： 67vs. 120 cells/field,P〈0.01; DU145： 130vs. 220 cells/field,P〈0.01）. The results of wound healing assay indicated that miR-30c markedly reduced the migration of LNCaP and DU145 cells （LNCaP： 241vs. 520 cells/field,P〈0.01; DU145： 490vs. 660 cells/field,P〈0.01）.ConclusionmiR-30c can inhibit invasion and migration of prostate cancer cells, which proved miR-30c can serve as tumor suppressor, and KRAS-MAPK pathway may be involved.